Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26610 | 3' | -55.5 | NC_005357.1 | + | 4922 | 0.66 | 0.603332 |
Target: 5'- cGGCGACgacCaGCUUCuuGCCCucGGGCg -3' miRNA: 3'- -CCGCUGaa-GcCGGAGuuCGGGu-UCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 22247 | 0.66 | 0.63702 |
Target: 5'- gGGCGACgacaaGGCg--AAGCCgCAAGGCg -3' miRNA: 3'- -CCGCUGaag--CCGgagUUCGG-GUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 30148 | 0.66 | 0.614549 |
Target: 5'- cGGCacGCUg-GGCCagCAAcccGCCCAGGACa -3' miRNA: 3'- -CCGc-UGAagCCGGa-GUU---CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 31710 | 0.66 | 0.601092 |
Target: 5'- aGGCGACgacgcauucaagcaaUCGGCCg-AGGCCgugcgcgcugccguCAAGACg -3' miRNA: 3'- -CCGCUGa--------------AGCCGGagUUCGG--------------GUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8134 | 0.66 | 0.614549 |
Target: 5'- cGGUGACUUCccaGCCcuugccguUCAccucgaugaugcGGCCCAGGAa -3' miRNA: 3'- -CCGCUGAAGc--CGG--------AGU------------UCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 18473 | 0.66 | 0.63702 |
Target: 5'- cGGCGGCgUCGGCCgauucGUCCAccguGAUg -3' miRNA: 3'- -CCGCUGaAGCCGGaguu-CGGGUu---CUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 27839 | 0.66 | 0.625782 |
Target: 5'- cGGUGGCgcCGGCCgcgCcgcuGCUCAAGAa -3' miRNA: 3'- -CCGCUGaaGCCGGa--Guu--CGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8452 | 0.66 | 0.63702 |
Target: 5'- cGCGAUgUCGGCgUCGagguGGCCUgcGAUg -3' miRNA: 3'- cCGCUGaAGCCGgAGU----UCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 2487 | 0.67 | 0.536889 |
Target: 5'- aGGCGGuCUUUGaaUUCGAGCgCCGAGAUu -3' miRNA: 3'- -CCGCU-GAAGCcgGAGUUCG-GGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 17836 | 0.67 | 0.533626 |
Target: 5'- gGGCGGgUUgaugcccgccaccgUGGCCUCGucGCCCAgguAGGCc -3' miRNA: 3'- -CCGCUgAA--------------GCCGGAGUu-CGGGU---UCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 33085 | 0.67 | 0.547818 |
Target: 5'- aGCGACgcggCGGCCUaCAuccucGCCCAuGAa -3' miRNA: 3'- cCGCUGaa--GCCGGA-GUu----CGGGUuCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 29056 | 0.67 | 0.530369 |
Target: 5'- cGGCGGCUcguaGGCCaucagcgggucauccUCGGGCgCCGAGGu -3' miRNA: 3'- -CCGCUGAag--CCGG---------------AGUUCG-GGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 20096 | 0.67 | 0.523877 |
Target: 5'- uGGCGACUUCGGaaaaggcGCCCAGcACa -3' miRNA: 3'- -CCGCUGAAGCCggaguu-CGGGUUcUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 37122 | 0.67 | 0.547818 |
Target: 5'- cGCGGCcuaCGGCCUCGAccuGCC--AGACa -3' miRNA: 3'- cCGCUGaa-GCCGGAGUU---CGGguUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 9756 | 0.67 | 0.558816 |
Target: 5'- gGGCGcGCUUC-GCCUCgAAGUCCA-GAUc -3' miRNA: 3'- -CCGC-UGAAGcCGGAG-UUCGGGUuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 4047 | 0.67 | 0.569874 |
Target: 5'- cGCGGCgagCGGCaggcCAgcGGCCCAGGGg -3' miRNA: 3'- cCGCUGaa-GCCGga--GU--UCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 41775 | 0.67 | 0.569874 |
Target: 5'- uGGUGGCUaCaGCCUUggGCaCCGAcucGACg -3' miRNA: 3'- -CCGCUGAaGcCGGAGuuCG-GGUU---CUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 10283 | 0.67 | 0.526038 |
Target: 5'- aGGuCGAgUUCGGCCUUGAGggauUCgGGGGCg -3' miRNA: 3'- -CC-GCUgAAGCCGGAGUUC----GGgUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 12911 | 0.67 | 0.580986 |
Target: 5'- uGGCGAUgcgcUCGGCCgc--GCCCAuaACg -3' miRNA: 3'- -CCGCUGa---AGCCGGaguuCGGGUucUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 1315 | 0.67 | 0.558816 |
Target: 5'- gGGCGACggccaccaGGCCgaCAAGCgCGAGGa -3' miRNA: 3'- -CCGCUGaag-----CCGGa-GUUCGgGUUCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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