Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26610 | 3' | -55.5 | NC_005357.1 | + | 340 | 0.69 | 0.409654 |
Target: 5'- cGGUGGCgucgcgcagcaGGCCguccgcCAGGCCCGAGAa -3' miRNA: 3'- -CCGCUGaag--------CCGGa-----GUUCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 616 | 0.7 | 0.367419 |
Target: 5'- cGGCGAUgaUGGCCUC-AGCCUc-GGCg -3' miRNA: 3'- -CCGCUGaaGCCGGAGuUCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 637 | 0.69 | 0.432856 |
Target: 5'- cGGCGuc--CGGCgUCGAgucgguGCCCAAGGCu -3' miRNA: 3'- -CCGCugaaGCCGgAGUU------CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 688 | 0.77 | 0.129869 |
Target: 5'- cGGCGGCgcCGGCCgggccGCCCAAGAUa -3' miRNA: 3'- -CCGCUGaaGCCGGaguu-CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 1315 | 0.67 | 0.558816 |
Target: 5'- gGGCGACggccaccaGGCCgaCAAGCgCGAGGa -3' miRNA: 3'- -CCGCUGaag-----CCGGa-GUUCGgGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 2487 | 0.67 | 0.536889 |
Target: 5'- aGGCGGuCUUUGaaUUCGAGCgCCGAGAUu -3' miRNA: 3'- -CCGCU-GAAGCcgGAGUUCG-GGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 4047 | 0.67 | 0.569874 |
Target: 5'- cGCGGCgagCGGCaggcCAgcGGCCCAGGGg -3' miRNA: 3'- cCGCUGaa-GCCGga--GU--UCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 4888 | 0.74 | 0.214232 |
Target: 5'- cGGCGACggCGGCCUCGuuuucGGCCaCGguGGAa -3' miRNA: 3'- -CCGCUGaaGCCGGAGU-----UCGG-GU--UCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 4922 | 0.66 | 0.603332 |
Target: 5'- cGGCGACgacCaGCUUCuuGCCCucGGGCg -3' miRNA: 3'- -CCGCUGaa-GcCGGAGuuCGGGu-UCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7247 | 0.7 | 0.394622 |
Target: 5'- cGGCGAUgUCGGCC----GCCCAGGuCu -3' miRNA: 3'- -CCGCUGaAGCCGGaguuCGGGUUCuG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7447 | 0.69 | 0.410605 |
Target: 5'- -uCGGCUUCGGCCUUcgcgcgaauggccuuGCaCCAGGACu -3' miRNA: 3'- ccGCUGAAGCCGGAGuu-------------CG-GGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7685 | 0.69 | 0.423095 |
Target: 5'- uGGCGGCgaucugCGGCCgguAGCCCucGAa -3' miRNA: 3'- -CCGCUGaa----GCCGGaguUCGGGuuCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8134 | 0.66 | 0.614549 |
Target: 5'- cGGUGACUUCccaGCCcuugccguUCAccucgaugaugcGGCCCAGGAa -3' miRNA: 3'- -CCGCUGAAGc--CGG--------AGU------------UCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8452 | 0.66 | 0.63702 |
Target: 5'- cGCGAUgUCGGCgUCGagguGGCCUgcGAUg -3' miRNA: 3'- cCGCUGaAGCCGgAGU----UCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8818 | 0.7 | 0.362135 |
Target: 5'- aGGCGguACUuggugUCGGCCUugauaccguccucgcCGacguAGCCCAGGACg -3' miRNA: 3'- -CCGC--UGA-----AGCCGGA---------------GU----UCGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 9313 | 0.72 | 0.309111 |
Target: 5'- cGGCGACaUUCagGGCCUUggGUUCAuGGGCg -3' miRNA: 3'- -CCGCUG-AAG--CCGGAGuuCGGGU-UCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 9756 | 0.67 | 0.558816 |
Target: 5'- gGGCGcGCUUC-GCCUCgAAGUCCA-GAUc -3' miRNA: 3'- -CCGC-UGAAGcCGGAG-UUCGGGUuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 9810 | 0.71 | 0.341533 |
Target: 5'- aGCGACcgCGGCgUCGcGGCCCuuGACg -3' miRNA: 3'- cCGCUGaaGCCGgAGU-UCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 10283 | 0.67 | 0.526038 |
Target: 5'- aGGuCGAgUUCGGCCUUGAGggauUCgGGGGCg -3' miRNA: 3'- -CC-GCUgAAGCCGGAGUUC----GGgUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 10765 | 0.71 | 0.340693 |
Target: 5'- -uCGACUUCGGCCcgCAguucuucGGCCUggGAUu -3' miRNA: 3'- ccGCUGAAGCCGGa-GU-------UCGGGuuCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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