Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26610 | 3' | -55.5 | NC_005357.1 | + | 37878 | 1.11 | 0.000459 |
Target: 5'- cGGCGACUUCGGCCUCAAGCCCAAGACc -3' miRNA: 3'- -CCGCUGAAGCCGGAGUUCGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 36234 | 0.81 | 0.072729 |
Target: 5'- cGGCGGCUaUCGGCUUCGGGuUCCAAGAg -3' miRNA: 3'- -CCGCUGA-AGCCGGAGUUC-GGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 12307 | 0.8 | 0.083722 |
Target: 5'- cGGCGGCguguucuggcgCGGCCUCAucGCCUggGACa -3' miRNA: 3'- -CCGCUGaa---------GCCGGAGUu-CGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 11407 | 0.79 | 0.100278 |
Target: 5'- cGCGAUUgccUCGGCCggCAAGCCCGuGGACa -3' miRNA: 3'- cCGCUGA---AGCCGGa-GUUCGGGU-UCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 688 | 0.77 | 0.129869 |
Target: 5'- cGGCGGCgcCGGCCgggccGCCCAAGAUa -3' miRNA: 3'- -CCGCUGaaGCCGGaguu-CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 15158 | 0.74 | 0.213654 |
Target: 5'- cGGCGaACUgcgguucauugccUCGGCCaaGAGCCUggGGCg -3' miRNA: 3'- -CCGC-UGA-------------AGCCGGagUUCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 4888 | 0.74 | 0.214232 |
Target: 5'- cGGCGACggCGGCCUCGuuuucGGCCaCGguGGAa -3' miRNA: 3'- -CCGCUGaaGCCGGAGU-----UCGG-GU--UCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 36148 | 0.72 | 0.286371 |
Target: 5'- gGGCGACcugUUCGGCCU---GCCCAGcGCg -3' miRNA: 3'- -CCGCUG---AAGCCGGAguuCGGGUUcUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 12616 | 0.72 | 0.301381 |
Target: 5'- uGGuCGACgauucaUCGGCaUCGAGCgCGAGACa -3' miRNA: 3'- -CC-GCUGa-----AGCCGgAGUUCGgGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 9313 | 0.72 | 0.309111 |
Target: 5'- cGGCGACaUUCagGGCCUUggGUUCAuGGGCg -3' miRNA: 3'- -CCGCUG-AAG--CCGGAGuuCGGGU-UCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 33852 | 0.71 | 0.325022 |
Target: 5'- aGGcCGcACgcgCGGCCUaCGAcGCCCAGGAUg -3' miRNA: 3'- -CC-GC-UGaa-GCCGGA-GUU-CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 10765 | 0.71 | 0.340693 |
Target: 5'- -uCGACUUCGGCCcgCAguucuucGGCCUggGAUu -3' miRNA: 3'- ccGCUGAAGCCGGa-GU-------UCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 9810 | 0.71 | 0.341533 |
Target: 5'- aGCGACcgCGGCgUCGcGGCCCuuGACg -3' miRNA: 3'- cCGCUGaaGCCGgAGU-UCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8818 | 0.7 | 0.362135 |
Target: 5'- aGGCGguACUuggugUCGGCCUugauaccguccucgcCGacguAGCCCAGGACg -3' miRNA: 3'- -CCGC--UGA-----AGCCGGA---------------GU----UCGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 616 | 0.7 | 0.367419 |
Target: 5'- cGGCGAUgaUGGCCUC-AGCCUc-GGCg -3' miRNA: 3'- -CCGCUGaaGCCGGAGuUCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 23215 | 0.7 | 0.385411 |
Target: 5'- cGGUGAaUUCGGCCUCGgccucGGCCaccGACc -3' miRNA: 3'- -CCGCUgAAGCCGGAGU-----UCGGguuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7247 | 0.7 | 0.394622 |
Target: 5'- cGGCGAUgUCGGCC----GCCCAGGuCu -3' miRNA: 3'- -CCGCUGaAGCCGGaguuCGGGUUCuG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 35183 | 0.7 | 0.401155 |
Target: 5'- cGGCGACaUCGGCC-CGcGCCaguggccgaaguugCAGGGCu -3' miRNA: 3'- -CCGCUGaAGCCGGaGUuCGG--------------GUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 340 | 0.69 | 0.409654 |
Target: 5'- cGGUGGCgucgcgcagcaGGCCguccgcCAGGCCCGAGAa -3' miRNA: 3'- -CCGCUGaag--------CCGGa-----GUUCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7447 | 0.69 | 0.410605 |
Target: 5'- -uCGGCUUCGGCCUUcgcgcgaauggccuuGCaCCAGGACu -3' miRNA: 3'- ccGCUGAAGCCGGAGuu-------------CG-GGUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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