Results 1 - 20 of 97 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26616 | 3' | -62.4 | NC_005357.1 | + | 39846 | 1.1 | 0.000115 |
Target: 5'- aACAGGGCGGCAAGCGCGCGCACGCGCu -3' miRNA: 3'- -UGUCCCGCCGUUCGCGCGCGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 2406 | 0.82 | 0.019698 |
Target: 5'- uCAGGGCGGCAAGCGUagGUGUACG-GCa -3' miRNA: 3'- uGUCCCGCCGUUCGCG--CGCGUGCgCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 38576 | 0.8 | 0.025556 |
Target: 5'- aGCuGGGCGGCGAG-GUGCGCAaagUGCGCu -3' miRNA: 3'- -UGuCCCGCCGUUCgCGCGCGU---GCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 33029 | 0.77 | 0.044132 |
Target: 5'- uGCuGGGCGGCAAGUgcaaguucccGCGUGCuguCGUGCg -3' miRNA: 3'- -UGuCCCGCCGUUCG----------CGCGCGu--GCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 25767 | 0.76 | 0.052375 |
Target: 5'- uGCuGGGCGGCGgcaacgauGGCcgcgauGCGCGCGgCGCGCu -3' miRNA: 3'- -UGuCCCGCCGU--------UCG------CGCGCGU-GCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 7058 | 0.75 | 0.06353 |
Target: 5'- cCGGGGCGGCcagguacuucaugacGccgcggccuacccacGGCGCGCcCACGCGCa -3' miRNA: 3'- uGUCCCGCCG---------------U---------------UCGCGCGcGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 26046 | 0.74 | 0.073576 |
Target: 5'- aGCccGcGCGGCGGGCGCGCucCAUGCGCu -3' miRNA: 3'- -UGucC-CGCCGUUCGCGCGc-GUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 14593 | 0.74 | 0.075676 |
Target: 5'- aGCGGcGCGGCcGGCGCcaccgcggGCaGCGCGCGCg -3' miRNA: 3'- -UGUCcCGCCGuUCGCG--------CG-CGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 24274 | 0.74 | 0.080048 |
Target: 5'- aACAGGGCuauuucGGCAAGCuGCGCGagcCGgGCg -3' miRNA: 3'- -UGUCCCG------CCGUUCG-CGCGCgu-GCgCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 12125 | 0.73 | 0.084662 |
Target: 5'- gGCAGGuuGGCGcGaCGCGC-CACGCGCu -3' miRNA: 3'- -UGUCCcgCCGUuC-GCGCGcGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 16173 | 0.73 | 0.089528 |
Target: 5'- cCAGGcCGGCGGGUGCccgugccuucgGCGaCGCGCGCa -3' miRNA: 3'- uGUCCcGCCGUUCGCG-----------CGC-GUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 32417 | 0.73 | 0.09206 |
Target: 5'- cCAGGGCGGgGGcCGgGCGCGCG-GCa -3' miRNA: 3'- uGUCCCGCCgUUcGCgCGCGUGCgCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 40057 | 0.73 | 0.09466 |
Target: 5'- cACGcGGGCGucugccacguGCAGGCcgaauuGCgGCGCACGCGCg -3' miRNA: 3'- -UGU-CCCGC----------CGUUCG------CG-CGCGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 12993 | 0.73 | 0.09466 |
Target: 5'- aGCAccuGGGCGcgacguGCGAGCGCGUGggcaACGCGCc -3' miRNA: 3'- -UGU---CCCGC------CGUUCGCGCGCg---UGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 24628 | 0.72 | 0.100069 |
Target: 5'- uGCA-GGCGGaauacuGCGCGCGCuucuauccguucGCGCGCg -3' miRNA: 3'- -UGUcCCGCCguu---CGCGCGCG------------UGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 14724 | 0.72 | 0.102882 |
Target: 5'- ---cGGCGGCGAGC-CGCuGCcgGCGCGCg -3' miRNA: 3'- ugucCCGCCGUUCGcGCG-CG--UGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 5612 | 0.72 | 0.105769 |
Target: 5'- gGCGGGGUGcGUuucGCGCGUGgCGCgGCGCa -3' miRNA: 3'- -UGUCCCGC-CGuu-CGCGCGC-GUG-CGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 2570 | 0.72 | 0.105769 |
Target: 5'- gACA---CGGCccAGCGCGUGCGCGCGCu -3' miRNA: 3'- -UGUcccGCCGu-UCGCGCGCGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 8663 | 0.72 | 0.114892 |
Target: 5'- cGCGcGGuCGGCAuuGCGUGC-CGCGCGCa -3' miRNA: 3'- -UGUcCC-GCCGUu-CGCGCGcGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 14993 | 0.72 | 0.114892 |
Target: 5'- cCAGGGCcugcGGCcgccGGGCGCgguGCGCcagGCGCGCg -3' miRNA: 3'- uGUCCCG----CCG----UUCGCG---CGCG---UGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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