Results 1 - 20 of 97 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26616 | 3' | -62.4 | NC_005357.1 | + | 392 | 0.69 | 0.177087 |
Target: 5'- -gAGcGCGGCcAGCGCGCgacuguugagcaGCACGuCGCg -3' miRNA: 3'- ugUCcCGCCGuUCGCGCG------------CGUGC-GCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 813 | 0.68 | 0.217752 |
Target: 5'- aGCA-GGUGGCugggcuuGAGCGCGCGCAgagacaGCGa -3' miRNA: 3'- -UGUcCCGCCG-------UUCGCGCGCGUg-----CGCg -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 1364 | 0.67 | 0.241856 |
Target: 5'- -aAGGGaUGGUgacauaGGGCGcCGCGgcCACGCGCa -3' miRNA: 3'- ugUCCC-GCCG------UUCGC-GCGC--GUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 1749 | 0.67 | 0.235779 |
Target: 5'- cACcGGGCGGUcauGGCcggccuGCGC-CugGCGCg -3' miRNA: 3'- -UGuCCCGCCGu--UCG------CGCGcGugCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 2235 | 0.68 | 0.218315 |
Target: 5'- cGCAGcaGGCGGC--GCGUG-GC-CGCGCa -3' miRNA: 3'- -UGUC--CCGCCGuuCGCGCgCGuGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 2406 | 0.82 | 0.019698 |
Target: 5'- uCAGGGCGGCAAGCGUagGUGUACG-GCa -3' miRNA: 3'- uGUCCCGCCGUUCGCG--CGCGUGCgCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 2570 | 0.72 | 0.105769 |
Target: 5'- gACA---CGGCccAGCGCGUGCGCGCGCu -3' miRNA: 3'- -UGUcccGCCGu-UCGCGCGCGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 4364 | 0.67 | 0.235779 |
Target: 5'- gGCcGGaCGGCAGGCGgaUGCGCAgccaggcgccguCGCGCc -3' miRNA: 3'- -UGuCCcGCCGUUCGC--GCGCGU------------GCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 4512 | 0.67 | 0.248063 |
Target: 5'- --cGGGCGGCGcccgugaacguGGCGUgcuggGCGguCGUGCc -3' miRNA: 3'- uguCCCGCCGU-----------UCGCG-----CGCguGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 4643 | 0.71 | 0.130674 |
Target: 5'- cGCAGGGuCGGCAAGaUcggacagguuguugGCGgGCACcaGCGCg -3' miRNA: 3'- -UGUCCC-GCCGUUC-G--------------CGCgCGUG--CGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 5094 | 0.67 | 0.260871 |
Target: 5'- aACA-GGCGGCcGGCccagcguccgGUGCgGCugGCGCc -3' miRNA: 3'- -UGUcCCGCCGuUCG----------CGCG-CGugCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 5612 | 0.72 | 0.105769 |
Target: 5'- gGCGGGGUGcGUuucGCGCGUGgCGCgGCGCa -3' miRNA: 3'- -UGUCCCGC-CGuu-CGCGCGC-GUG-CGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 6622 | 0.66 | 0.274212 |
Target: 5'- aGCGGGcgcGUGGCGcuGCGCGCGgACaCGUa -3' miRNA: 3'- -UGUCC---CGCCGUu-CGCGCGCgUGcGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 7058 | 0.75 | 0.06353 |
Target: 5'- cCGGGGCGGCcagguacuucaugacGccgcggccuacccacGGCGCGCcCACGCGCa -3' miRNA: 3'- uGUCCCGCCG---------------U---------------UCGCGCGcGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 7065 | 0.66 | 0.274212 |
Target: 5'- --cGGGCGGC-AGCGuCGUuugaacagGCugGCGg -3' miRNA: 3'- uguCCCGCCGuUCGC-GCG--------CGugCGCg -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 7414 | 0.69 | 0.183765 |
Target: 5'- gACAGGGUauaGaGC-AGCGgGCGCccggacacuuccucgACGCGCg -3' miRNA: 3'- -UGUCCCG---C-CGuUCGCgCGCG---------------UGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 7830 | 0.67 | 0.254401 |
Target: 5'- -gAGGcGCGGCugcaCGaugaccagGCGCACGCGCu -3' miRNA: 3'- ugUCC-CGCCGuuc-GCg-------CGCGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 7892 | 0.71 | 0.121378 |
Target: 5'- cCAGGGCGGC--GCGUGCGUAgauCGCcaGCu -3' miRNA: 3'- uGUCCCGCCGuuCGCGCGCGU---GCG--CG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 8143 | 0.68 | 0.22401 |
Target: 5'- --uGGGCGuGCGGGUgucgGCGCugcgucccuGCGCGUGCg -3' miRNA: 3'- uguCCCGC-CGUUCG----CGCG---------CGUGCGCG- -5' |
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26616 | 3' | -62.4 | NC_005357.1 | + | 8211 | 0.67 | 0.235779 |
Target: 5'- gACAuGGCGGCcAGUucGUGCGCGgcCGUGCc -3' miRNA: 3'- -UGUcCCGCCGuUCG--CGCGCGU--GCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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