Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26648 | 3' | -51.8 | NC_005808.1 | + | 25381 | 0.66 | 0.852919 |
Target: 5'- --cCGgCGACGAGCgccggUGGCaGGGCCGg -3' miRNA: 3'- ugaGCgGCUGUUUGa----AUCGgCUUGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 31024 | 0.66 | 0.852919 |
Target: 5'- gGCUUGCCGGCcgAGGCaaucGCgCGGGCCa -3' miRNA: 3'- -UGAGCGGCUG--UUUGaau-CG-GCUUGGc -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 32707 | 0.66 | 0.852919 |
Target: 5'- uGCUCGCCGcgugcaauCAGGCgauggagGGCUGAcGCCa -3' miRNA: 3'- -UGAGCGGCu-------GUUUGaa-----UCGGCU-UGGc -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 679 | 0.66 | 0.844016 |
Target: 5'- gGC-CGCCuACGgcGGCgccGGCCGGGCCGc -3' miRNA: 3'- -UGaGCGGcUGU--UUGaa-UCGGCUUGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 26071 | 0.66 | 0.844016 |
Target: 5'- --gCGCuCGGCGAACUcgGGgCGGGCCu -3' miRNA: 3'- ugaGCG-GCUGUUUGAa-UCgGCUUGGc -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 23759 | 0.66 | 0.844016 |
Target: 5'- --aUGCCGGCGccACcgGGCCGAugCGc -3' miRNA: 3'- ugaGCGGCUGUu-UGaaUCGGCUugGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 37989 | 0.66 | 0.844016 |
Target: 5'- --cCGCCGACAucauc-GCCGAgGCCGa -3' miRNA: 3'- ugaGCGGCUGUuugaauCGGCU-UGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 31311 | 0.66 | 0.844016 |
Target: 5'- -gUCGcCCGGCucguAGCUgGGCCGcgcGCCGg -3' miRNA: 3'- ugAGC-GGCUGu---UUGAaUCGGCu--UGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 18465 | 0.66 | 0.843113 |
Target: 5'- cGCggCGCCGGC-GGCgucGGCCGAuucguccACCGu -3' miRNA: 3'- -UGa-GCGGCUGuUUGaa-UCGGCU-------UGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 31391 | 0.66 | 0.834872 |
Target: 5'- -gUCGCCG-CGAcCUgggauuccAGCCGGGCCa -3' miRNA: 3'- ugAGCGGCuGUUuGAa-------UCGGCUUGGc -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 33735 | 0.66 | 0.815901 |
Target: 5'- gAUUCGCCaGCGG---UGGCCGuGCCGg -3' miRNA: 3'- -UGAGCGGcUGUUugaAUCGGCuUGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 14956 | 0.66 | 0.815901 |
Target: 5'- -aUCGCCGcGCAGACccugaaAGCCGGGCa- -3' miRNA: 3'- ugAGCGGC-UGUUUGaa----UCGGCUUGgc -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 33711 | 0.66 | 0.815901 |
Target: 5'- gAUUCGCCaGCGG---UGGCCGuGCCGg -3' miRNA: 3'- -UGAGCGGcUGUUugaAUCGGCuUGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 24395 | 0.66 | 0.806096 |
Target: 5'- aACUCGCUGcGCAAguugaagaaGCgcAGCCGGcCCGa -3' miRNA: 3'- -UGAGCGGC-UGUU---------UGaaUCGGCUuGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 9360 | 0.66 | 0.805104 |
Target: 5'- -gUCGCCGAgGAagucaauGCgcAGCCGGuguACCGg -3' miRNA: 3'- ugAGCGGCUgUU-------UGaaUCGGCU---UGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 37344 | 0.67 | 0.796094 |
Target: 5'- cGCaUUGCCGACcccGACcugccGGCCGAACUGc -3' miRNA: 3'- -UG-AGCGGCUGu--UUGaa---UCGGCUUGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 12083 | 0.67 | 0.796094 |
Target: 5'- cCUCGCCGGCcacggccgcGCUgcUGGCCGAcguggaaaagGCCa -3' miRNA: 3'- uGAGCGGCUGuu-------UGA--AUCGGCU----------UGGc -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 18578 | 0.67 | 0.796094 |
Target: 5'- cGCUCGCgGugAcGGCcUGGCCGu-CCGa -3' miRNA: 3'- -UGAGCGgCugU-UUGaAUCGGCuuGGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 25711 | 0.67 | 0.785906 |
Target: 5'- --aCGCCGGCAGAacu-GCCGAGgUGg -3' miRNA: 3'- ugaGCGGCUGUUUgaauCGGCUUgGC- -5' |
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26648 | 3' | -51.8 | NC_005808.1 | + | 17725 | 0.67 | 0.785906 |
Target: 5'- aGCcCGCCGACGcaucaaGAUcgUGGCCGuacuGCCGc -3' miRNA: 3'- -UGaGCGGCUGU------UUGa-AUCGGCu---UGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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