Results 1 - 20 of 59 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26653 | 3' | -56.4 | NC_005808.1 | + | 17897 | 0.66 | 0.586638 |
Target: 5'- -gUGGCGGGCaucaaCC-CGCCCGAGGg -3' miRNA: 3'- aaGCCGCUUGaca--GGuGCGGGCUUCg -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 7496 | 0.66 | 0.586638 |
Target: 5'- gUCGaG-GAAgUGUCCggGCGCCCGcuGCu -3' miRNA: 3'- aAGC-CgCUUgACAGG--UGCGGGCuuCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 9100 | 0.66 | 0.579992 |
Target: 5'- gUUCGGCuuuacgucuggugacGAACUGgugcgCCGCcuGCUgGAAGCg -3' miRNA: 3'- -AAGCCG---------------CUUGACa----GGUG--CGGgCUUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 39342 | 0.66 | 0.57557 |
Target: 5'- -cUGGCGcggaAGCUGaaUCC-CGCCgGggGCu -3' miRNA: 3'- aaGCCGC----UUGAC--AGGuGCGGgCuuCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 34585 | 0.66 | 0.57557 |
Target: 5'- -cCGGCGaAGCUGgcgCCaagggcACGUCCGAcGCc -3' miRNA: 3'- aaGCCGC-UUGACa--GG------UGCGGGCUuCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 14817 | 0.66 | 0.57557 |
Target: 5'- cUCGGCGGcGCUG-CCuuCGCCC-AGGUc -3' miRNA: 3'- aAGCCGCU-UGACaGGu-GCGGGcUUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 27417 | 0.66 | 0.564549 |
Target: 5'- gUCGcGCGc-CUGgcgcaCCGCGCCCGGcGGCc -3' miRNA: 3'- aAGC-CGCuuGACa----GGUGCGGGCU-UCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 10367 | 0.66 | 0.564549 |
Target: 5'- -cUGGCGAAgUGg-CAC-CCCGAGGUg -3' miRNA: 3'- aaGCCGCUUgACagGUGcGGGCUUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 804 | 0.66 | 0.553584 |
Target: 5'- -cCGGCGAcGCg--CCGCGCCC--AGCc -3' miRNA: 3'- aaGCCGCU-UGacaGGUGCGGGcuUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 26053 | 0.66 | 0.553584 |
Target: 5'- -gCGGCGGGCgcgcUCCAUGCgcucggcgaacUCGggGCg -3' miRNA: 3'- aaGCCGCUUGac--AGGUGCG-----------GGCuuCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 35269 | 0.66 | 0.553584 |
Target: 5'- -gCGGCGAcCU-UCCACcaGCuuGAAGCc -3' miRNA: 3'- aaGCCGCUuGAcAGGUG--CGggCUUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 37055 | 0.66 | 0.553584 |
Target: 5'- cUCGGCGGGgUGcguUUCGCGCgUGgcGCg -3' miRNA: 3'- aAGCCGCUUgAC---AGGUGCGgGCuuCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 30393 | 0.66 | 0.550307 |
Target: 5'- gUCGGUGAuaACggcGUCCACGCUuucgcucggcauggCGAugAGCg -3' miRNA: 3'- aAGCCGCU--UGa--CAGGUGCGG--------------GCU--UCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 9665 | 0.66 | 0.542684 |
Target: 5'- -aCGuCGAACU---CACGCCCGAGGUg -3' miRNA: 3'- aaGCcGCUUGAcagGUGCGGGCUUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 28379 | 0.66 | 0.541598 |
Target: 5'- cUUGGCGAGgUGUUCcaguucgACGCCgGcGGCg -3' miRNA: 3'- aAGCCGCUUgACAGG-------UGCGGgCuUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 15723 | 0.66 | 0.541597 |
Target: 5'- cUCGGgGAGCcGguaagCCAUaucagcgGCCUGGAGCa -3' miRNA: 3'- aAGCCgCUUGaCa----GGUG-------CGGGCUUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 27847 | 0.67 | 0.536178 |
Target: 5'- -cCGGCcgcgccgcugcucaaGAACgUGUCCGCGCUCGccaucuuGGCg -3' miRNA: 3'- aaGCCG---------------CUUG-ACAGGUGCGGGCu------UCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 13647 | 0.67 | 0.531855 |
Target: 5'- -cCGGCGcGGCcGUCCAUGUCCuuGGUg -3' miRNA: 3'- aaGCCGC-UUGaCAGGUGCGGGcuUCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 30614 | 0.67 | 0.531855 |
Target: 5'- aUCGGCGAuCUGUUCuuGCgaccagCCGgcGCg -3' miRNA: 3'- aAGCCGCUuGACAGGugCG------GGCuuCG- -5' |
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26653 | 3' | -56.4 | NC_005808.1 | + | 28203 | 0.67 | 0.510441 |
Target: 5'- -cCGGCaAGCUG-CUGcCGCCCGAccaGGCg -3' miRNA: 3'- aaGCCGcUUGACaGGU-GCGGGCU---UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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