Results 1 - 20 of 94 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26699 | 5' | -59.5 | NC_005808.1 | + | 42403 | 0.66 | 0.465739 |
Target: 5'- cCGUGucGCCCG-GC-AGCGUGACGguGCCc -3' miRNA: 3'- -GCGC--UGGGCuCGuUCGCGCUGC--UGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 42063 | 0.71 | 0.216168 |
Target: 5'- aGCGGCCCGcGCAcguccgagguauucGGCcaGCGAUGGCCc -3' miRNA: 3'- gCGCUGGGCuCGU--------------UCG--CGCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41914 | 0.66 | 0.426492 |
Target: 5'- gGCG-CCCG-GCcGGCGCcgccguaGGCGGCCu -3' miRNA: 3'- gCGCuGGGCuCGuUCGCG-------CUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41789 | 0.66 | 0.446353 |
Target: 5'- -uCGGCaaGGGCuGGGCGCGGCGcgucGCCGg -3' miRNA: 3'- gcGCUGggCUCG-UUCGCGCUGC----UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41647 | 0.66 | 0.427426 |
Target: 5'- aCGUGGCUCGAcacGCAgAGCGUGGagaauuCGACCa -3' miRNA: 3'- -GCGCUGGGCU---CGU-UCGCGCU------GCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41421 | 0.66 | 0.472626 |
Target: 5'- gGcCGACCCcGGCGgcgagacuauguggGGCGUGACcGCCGc -3' miRNA: 3'- gC-GCUGGGcUCGU--------------UCGCGCUGcUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41200 | 0.67 | 0.418142 |
Target: 5'- gGCGGCCaUGAGUGAaaccguuguGCGCGugG-CCGc -3' miRNA: 3'- gCGCUGG-GCUCGUU---------CGCGCugCuGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 40831 | 0.67 | 0.408982 |
Target: 5'- -uCGGCCCGAcGCGccaGGCGCaGGcCGGCCa -3' miRNA: 3'- gcGCUGGGCU-CGU---UCGCG-CU-GCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 40363 | 0.66 | 0.465739 |
Target: 5'- gCGCGGCCaCGcGCcgccugcuGCGCGugGugCu -3' miRNA: 3'- -GCGCUGG-GCuCGuu------CGCGCugCugGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 39779 | 0.7 | 0.253887 |
Target: 5'- gCGUGGCCgCGAGCAccuGGCcCGACGaAUCGa -3' miRNA: 3'- -GCGCUGG-GCUCGU---UCGcGCUGC-UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 39656 | 0.67 | 0.373625 |
Target: 5'- uCGaCGugCCGcGCGAcGCgGUGugGGCCGg -3' miRNA: 3'- -GC-GCugGGCuCGUU-CG-CGCugCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 38028 | 0.67 | 0.399948 |
Target: 5'- gGCG-CaCCGAGC-AGCGCcucGGCGACUu -3' miRNA: 3'- gCGCuG-GGCUCGuUCGCG---CUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 37819 | 0.67 | 0.418142 |
Target: 5'- gGCGugCUGGGCGcAGUGUgGGCGaacGCCGa -3' miRNA: 3'- gCGCugGGCUCGU-UCGCG-CUGC---UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 37218 | 0.66 | 0.464759 |
Target: 5'- gCGCGcaccagugacauaACCCuGGGCGcGGUGCaGGCGGCCa -3' miRNA: 3'- -GCGC-------------UGGG-CUCGU-UCGCG-CUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 37063 | 0.7 | 0.253887 |
Target: 5'- gGCGACCUGGGCGGaaccUG-GGCGGCCGg -3' miRNA: 3'- gCGCUGGGCUCGUUc---GCgCUGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 36198 | 0.68 | 0.356741 |
Target: 5'- uCGaCGACaucaCCGAGgGcGcCGCGGCGGCCGa -3' miRNA: 3'- -GC-GCUG----GGCUCgUuC-GCGCUGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 36112 | 0.69 | 0.309369 |
Target: 5'- gCGCGugaAUgCGGGCcuGCGCGGCGugCa -3' miRNA: 3'- -GCGC---UGgGCUCGuuCGCGCUGCugGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 36061 | 0.68 | 0.356741 |
Target: 5'- gGCGAUUCGGGCGA---CGACGACCc -3' miRNA: 3'- gCGCUGGGCUCGUUcgcGCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 35359 | 0.69 | 0.287542 |
Target: 5'- gGCGGCCgacaucgccgaGGGUGAGCGCGGCGcagaugcuGCCGu -3' miRNA: 3'- gCGCUGGg----------CUCGUUCGCGCUGC--------UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 34909 | 0.66 | 0.475594 |
Target: 5'- aGcCGGCcgCCGAGguCAAGC-CGACGACCu -3' miRNA: 3'- gC-GCUG--GGCUC--GUUCGcGCUGCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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