Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 26727 | 5' | -55.7 | NC_005808.1 | + | 22479 | 0.69 | 0.424173 |
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Target: 5'- aGGcgGAACcaggggcggGCUGGGCuGGUCAUGcccGCCa -3' miRNA: 3'- aUCuaCUUG---------UGGCCCG-CCAGUAC---CGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 1440 | 0.69 | 0.414532 |
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Target: 5'- aAGAUGGcaaACACCuucuugacGGcGCGGUCGgcGGCCu -3' miRNA: 3'- aUCUACU---UGUGG--------CC-CGCCAGUa-CCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 11023 | 0.7 | 0.386438 |
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Target: 5'- -cGAUGAacaccugcuGCACCGGGCcGUUcucGGCCg -3' miRNA: 3'- auCUACU---------UGUGGCCCGcCAGua-CCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 37836 | 0.73 | 0.252212 |
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Target: 5'- gUGGgcGAACGCCGagaacaucGCGGcCGUGGCCg -3' miRNA: 3'- -AUCuaCUUGUGGCc-------CGCCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 17910 | 0.73 | 0.258922 |
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Target: 5'- -cGAUGGACAgcCUGGGCguGGaCGUGGCCc -3' miRNA: 3'- auCUACUUGU--GGCCCG--CCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 29612 | 0.73 | 0.232939 |
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Target: 5'- cAGuUGuccAGCGCCGGGCGcUUGUGGCCg -3' miRNA: 3'- aUCuAC---UUGUGGCCCGCcAGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 4523 | 0.74 | 0.20303 |
|
Target: 5'- --cGUGAACguggcguGCUGGGCGGUCGUGccGCCg -3' miRNA: 3'- aucUACUUG-------UGGCCCGCCAGUAC--CGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 1740 | 1.11 | 0.000448 |
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Target: 5'- gUAGAUGAACACCGGGCGGUCAUGGCCg -3' miRNA: 3'- -AUCUACUUGUGGCCCGCCAGUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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