Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26727 | 5' | -55.7 | NC_005808.1 | + | 29041 | 0.66 | 0.626969 |
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Target: 5'- cAGGUcGAUACCcaccGGCGGcUCGUaGGCCa -3' miRNA: 3'- aUCUAcUUGUGGc---CCGCC-AGUA-CCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 29612 | 0.73 | 0.232939 |
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Target: 5'- cAGuUGuccAGCGCCGGGCGcUUGUGGCCg -3' miRNA: 3'- aUCuAC---UUGUGGCCCGCcAGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 31017 | 0.66 | 0.615739 |
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Target: 5'- cAGccGGcgGCcCUGGGCGG-CAUGGCUu -3' miRNA: 3'- aUCuaCU--UGuGGCCCGCCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 31376 | 0.66 | 0.626969 |
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Target: 5'- gGGcGUGGGCGggaaCGuGGCGGUCAUGcGCg -3' miRNA: 3'- aUC-UACUUGUg---GC-CCGCCAGUAC-CGg -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 34823 | 0.66 | 0.615739 |
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Target: 5'- aGGGUGGACGCCGGcGCaGaUCGc-GCCg -3' miRNA: 3'- aUCUACUUGUGGCC-CGcC-AGUacCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 37836 | 0.73 | 0.252212 |
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Target: 5'- gUGGgcGAACGCCGagaacaucGCGGcCGUGGCCg -3' miRNA: 3'- -AUCuaCUUGUGGCc-------CGCCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 39934 | 0.66 | 0.626969 |
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Target: 5'- -uGAUcGAACACCuGGGCGaccgC-UGGCCc -3' miRNA: 3'- auCUA-CUUGUGG-CCCGCca--GuACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 41606 | 0.66 | 0.593332 |
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Target: 5'- -cGGUGAaccacgGCACCGgccaGGCGGcCAagUGGCUg -3' miRNA: 3'- auCUACU------UGUGGC----CCGCCaGU--ACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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