Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26727 | 5' | -55.7 | NC_005808.1 | + | 41606 | 0.66 | 0.593332 |
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Target: 5'- -cGGUGAaccacgGCACCGgccaGGCGGcCAagUGGCUg -3' miRNA: 3'- auCUACU------UGUGGC----CCGCCaGU--ACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 7158 | 0.66 | 0.582175 |
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Target: 5'- cUGGAUGAccaGgGCCGGGCGcuuGUCGgauucgggGGCg -3' miRNA: 3'- -AUCUACU---UgUGGCCCGC---CAGUa-------CCGg -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 5825 | 0.66 | 0.571062 |
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Target: 5'- gGGcAUGGcgugccgcaGCACCGuGCGGUCgaaGUGGCUa -3' miRNA: 3'- aUC-UACU---------UGUGGCcCGCCAG---UACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 9861 | 0.67 | 0.527209 |
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Target: 5'- ---uUGGugG--GGGCGGUCAUGGCg -3' miRNA: 3'- aucuACUugUggCCCGCCAGUACCGg -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 6205 | 0.69 | 0.433946 |
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Target: 5'- cAGGUGAcgGCGCgCGGcuccUGGUCGUGGUCu -3' miRNA: 3'- aUCUACU--UGUG-GCCc---GCCAGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 17910 | 0.73 | 0.258922 |
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Target: 5'- -cGAUGGACAgcCUGGGCguGGaCGUGGCCc -3' miRNA: 3'- auCUACUUGU--GGCCCG--CCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 37836 | 0.73 | 0.252212 |
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Target: 5'- gUGGgcGAACGCCGagaacaucGCGGcCGUGGCCg -3' miRNA: 3'- -AUCuaCUUGUGGCc-------CGCCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 1740 | 1.11 | 0.000448 |
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Target: 5'- gUAGAUGAACACCGGGCGGUCAUGGCCg -3' miRNA: 3'- -AUCUACUUGUGGCCCGCCAGUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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