Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26727 | 5' | -55.7 | NC_005808.1 | + | 22768 | 0.66 | 0.575501 |
Target: 5'- aGGGUGAgggccgauuggucaACGCCcugcGGGcCGGUCAgcauggugccggacUGGCCc -3' miRNA: 3'- aUCUACU--------------UGUGG----CCC-GCCAGU--------------ACCGG- -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 2221 | 0.66 | 0.571061 |
Target: 5'- cGGGU-AGCACCacgcgcagcaGGCGGcgCGUGGCCg -3' miRNA: 3'- aUCUAcUUGUGGc---------CCGCCa-GUACCGG- -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 5460 | 0.66 | 0.571061 |
Target: 5'- -cGAUGuuccCGCCGGGCGG-CGaGGUg -3' miRNA: 3'- auCUACuu--GUGGCCCGCCaGUaCCGg -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 18855 | 0.67 | 0.538066 |
Target: 5'- ----aGAAgGCCGGGCGGccCAUGcCCa -3' miRNA: 3'- aucuaCUUgUGGCCCGCCa-GUACcGG- -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 22479 | 0.69 | 0.424173 |
Target: 5'- aGGcgGAACcaggggcggGCUGGGCuGGUCAUGcccGCCa -3' miRNA: 3'- aUCuaCUUG---------UGGCCCG-CCAGUAC---CGG- -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 1440 | 0.69 | 0.414532 |
Target: 5'- aAGAUGGcaaACACCuucuugacGGcGCGGUCGgcGGCCu -3' miRNA: 3'- aUCUACU---UGUGG--------CC-CGCCAGUa-CCGG- -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 11023 | 0.7 | 0.386438 |
Target: 5'- -cGAUGAacaccugcuGCACCGGGCcGUUcucGGCCg -3' miRNA: 3'- auCUACU---------UGUGGCCCGcCAGua-CCGG- -5' |
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26727 | 5' | -55.7 | NC_005808.1 | + | 29612 | 0.73 | 0.232939 |
Target: 5'- cAGuUGuccAGCGCCGGGCGcUUGUGGCCg -3' miRNA: 3'- aUCuAC---UUGUGGCCCGCcAGUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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