Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26727 | 5' | -55.7 | NC_005808.1 | + | 5460 | 0.66 | 0.571061 |
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Target: 5'- -cGAUGuuccCGCCGGGCGG-CGaGGUg -3' miRNA: 3'- auCUACuu--GUGGCCCGCCaGUaCCGg -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 2221 | 0.66 | 0.571061 |
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Target: 5'- cGGGU-AGCACCacgcgcagcaGGCGGcgCGUGGCCg -3' miRNA: 3'- aUCUAcUUGUGGc---------CCGCCa-GUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 22768 | 0.66 | 0.575501 |
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Target: 5'- aGGGUGAgggccgauuggucaACGCCcugcGGGcCGGUCAgcauggugccggacUGGCCc -3' miRNA: 3'- aUCUACU--------------UGUGG----CCC-GCCAGU--------------ACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 24567 | 0.66 | 0.593332 |
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Target: 5'- -cGAUGGcgAgGCCGGccaCGGUCG-GGCCg -3' miRNA: 3'- auCUACU--UgUGGCCc--GCCAGUaCCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 4258 | 0.66 | 0.615738 |
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Target: 5'- -cGGUGccggccgucAGCGCCGGcaUGGUgAUGGCCg -3' miRNA: 3'- auCUAC---------UUGUGGCCc-GCCAgUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 29041 | 0.66 | 0.626969 |
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Target: 5'- cAGGUcGAUACCcaccGGCGGcUCGUaGGCCa -3' miRNA: 3'- aUCUAcUUGUGGc---CCGCC-AGUA-CCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 39934 | 0.66 | 0.626969 |
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Target: 5'- -uGAUcGAACACCuGGGCGaccgC-UGGCCc -3' miRNA: 3'- auCUA-CUUGUGG-CCCGCca--GuACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 4523 | 0.74 | 0.20303 |
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Target: 5'- --cGUGAACguggcguGCUGGGCGGUCGUGccGCCg -3' miRNA: 3'- aucUACUUG-------UGGCCCGCCAGUAC--CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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