Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26727 | 5' | -55.7 | NC_005808.1 | + | 10213 | 0.66 | 0.615739 |
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Target: 5'- -cGGUGcuGGCuuccuGCUGGGCGGcCggGGCCu -3' miRNA: 3'- auCUAC--UUG-----UGGCCCGCCaGuaCCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 34823 | 0.66 | 0.615739 |
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Target: 5'- aGGGUGGACGCCGGcGCaGaUCGc-GCCg -3' miRNA: 3'- aUCUACUUGUGGCC-CGcC-AGUacCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 17287 | 0.66 | 0.615739 |
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Target: 5'- -cGAcGAGCGCC-GGUGG-CAgGGCCg -3' miRNA: 3'- auCUaCUUGUGGcCCGCCaGUaCCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 31017 | 0.66 | 0.615739 |
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Target: 5'- cAGccGGcgGCcCUGGGCGG-CAUGGCUu -3' miRNA: 3'- aUCuaCU--UGuGGCCCGCCaGUACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 31376 | 0.66 | 0.626969 |
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Target: 5'- gGGcGUGGGCGggaaCGuGGCGGUCAUGcGCg -3' miRNA: 3'- aUC-UACUUGUg---GC-CCGCCAGUAC-CGg -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 39934 | 0.66 | 0.626969 |
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Target: 5'- -uGAUcGAACACCuGGGCGaccgC-UGGCCc -3' miRNA: 3'- auCUA-CUUGUGG-CCCGCca--GuACCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 29041 | 0.66 | 0.626969 |
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Target: 5'- cAGGUcGAUACCcaccGGCGGcUCGUaGGCCa -3' miRNA: 3'- aUCUAcUUGUGGc---CCGCC-AGUA-CCGG- -5' |
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| 26727 | 5' | -55.7 | NC_005808.1 | + | 6614 | 0.66 | 0.626969 |
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Target: 5'- -cGAUGAcCAgCGGGCG--CGUGGCg -3' miRNA: 3'- auCUACUuGUgGCCCGCcaGUACCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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