Results 1 - 20 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26758 | 3' | -54.2 | NC_005808.1 | + | 5095 | 0.66 | 0.679213 |
Target: 5'- aCAGGCGGccGGCCCAgcguCCGGUgcggCUggCGCc -3' miRNA: 3'- cGUCCGCU--UUGGGU----GGUCAa---GGa-GCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 33866 | 0.66 | 0.723334 |
Target: 5'- gGUAGcGCcgcGCCCGCCGGgcacaUCUUCGUg -3' miRNA: 3'- -CGUC-CGcuuUGGGUGGUCa----AGGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 2515 | 0.66 | 0.723334 |
Target: 5'- uUAGGCGggGCUUcgguucguagACCAGgaaUUCGCg -3' miRNA: 3'- cGUCCGCuuUGGG----------UGGUCaagGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 6387 | 0.66 | 0.722246 |
Target: 5'- uCAGGCGcAGGCCgGCCGGc-CCgguguagUCGCg -3' miRNA: 3'- cGUCCGC-UUUGGgUGGUCaaGG-------AGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 10397 | 0.66 | 0.712415 |
Target: 5'- cCGGGUGAugcAGCgCGCCGauggCCUCGCc -3' miRNA: 3'- cGUCCGCU---UUGgGUGGUcaa-GGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 27592 | 0.66 | 0.712415 |
Target: 5'- aGCAGGCGgcGCCagaugGCguGgUCgUCGCc -3' miRNA: 3'- -CGUCCGCuuUGGg----UGguCaAGgAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 24338 | 0.66 | 0.705824 |
Target: 5'- gGCAGaGCGAuGCCCGuguuguuggucggguCgAGggCUUCGCg -3' miRNA: 3'- -CGUC-CGCUuUGGGU---------------GgUCaaGGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 35350 | 0.66 | 0.701414 |
Target: 5'- gGCcGGCGAcaucGGCCCgcGCCAGUggCCgaagUUGCa -3' miRNA: 3'- -CGuCCGCU----UUGGG--UGGUCAa-GG----AGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 26260 | 0.66 | 0.690343 |
Target: 5'- aCGGGC---ACCCGCCGGccuggUCggCUCGCg -3' miRNA: 3'- cGUCCGcuuUGGGUGGUCa----AG--GAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 1761 | 0.66 | 0.690343 |
Target: 5'- aCAGGCG-AugCCAUauuGUUCCUC-Ca -3' miRNA: 3'- cGUCCGCuUugGGUGgu-CAAGGAGcG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 1629 | 0.66 | 0.679213 |
Target: 5'- uCGGGCGugucguACCCAUCGGgguaggUCUgCGCg -3' miRNA: 3'- cGUCCGCuu----UGGGUGGUCa-----AGGaGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 26061 | 0.66 | 0.721158 |
Target: 5'- aGCaAGGCGGccuguacGGCuauaucggccagaCCACCGGcaCCUCGCu -3' miRNA: 3'- -CG-UCCGCU-------UUG-------------GGUGGUCaaGGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 30404 | 0.66 | 0.723334 |
Target: 5'- gGCAGGC--AGCgCggGCCGGUuucgUUCUCGCa -3' miRNA: 3'- -CGUCCGcuUUGgG--UGGUCA----AGGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 28192 | 0.66 | 0.723334 |
Target: 5'- cGCcuGGCGAcGCCCagcuucgcuacGCCuacGUUCCgcggCGCg -3' miRNA: 3'- -CGu-CCGCUuUGGG-----------UGGu--CAAGGa---GCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 654 | 0.66 | 0.701414 |
Target: 5'- uCAGGCGggGgcuuuugguguuUCUGCCgcGGUaCCUCGCu -3' miRNA: 3'- cGUCCGCuuU------------GGGUGG--UCAaGGAGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 11362 | 0.67 | 0.656824 |
Target: 5'- cGguGGCGAccuGCUCGCCcGGcaCCUCGg -3' miRNA: 3'- -CguCCGCUu--UGGGUGG-UCaaGGAGCg -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 13439 | 0.67 | 0.645588 |
Target: 5'- --cGGC-AGGCCCGCCAGcuuggcggCCUCGg -3' miRNA: 3'- cguCCGcUUUGGGUGGUCaa------GGAGCg -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 3480 | 0.67 | 0.644463 |
Target: 5'- cGCGGGCGAGuucgacgGCCCgcuguACCAGUaCgCagCGCg -3' miRNA: 3'- -CGUCCGCUU-------UGGG-----UGGUCAaG-Ga-GCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 16786 | 0.67 | 0.634339 |
Target: 5'- -aAGGCGAuGgUCGCCAGUUgCCaguuggUCGCg -3' miRNA: 3'- cgUCCGCUuUgGGUGGUCAA-GG------AGCG- -5' |
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26758 | 3' | -54.2 | NC_005808.1 | + | 1751 | 0.67 | 0.623088 |
Target: 5'- cCGGGCGGucauGGCCgGCCuGcgCCUgGCg -3' miRNA: 3'- cGUCCGCU----UUGGgUGGuCaaGGAgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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