Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27419 | 3' | -68.7 | NC_005869.1 | + | 8304 | 0.66 | 0.114836 |
Target: 5'- aGGUgGGCCCG-GCgGCgCGGGCCaGCu -3' miRNA: 3'- cUCGaCCGGGCgCGgCG-GCCCGGgUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 10626 | 0.66 | 0.108786 |
Target: 5'- -cGCcGGCCgGCGCC-CUGGGCgCGg -3' miRNA: 3'- cuCGaCCGGgCGCGGcGGCCCGgGUg -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 10710 | 0.66 | 0.108786 |
Target: 5'- aGGGcCUGGCgCG-GCUGUCGGGCgCgGCg -3' miRNA: 3'- -CUC-GACCGgGCgCGGCGGCCCG-GgUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 20625 | 0.66 | 0.105875 |
Target: 5'- aAGCUGGCCaCGgGCagGCCcaucuccuGGGCgCGCg -3' miRNA: 3'- cUCGACCGG-GCgCGg-CGG--------CCCGgGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 9122 | 0.66 | 0.105875 |
Target: 5'- gGAGUgGGCUCGCGCgGCgcaccagcaGGGUCUGCa -3' miRNA: 3'- -CUCGaCCGGGCGCGgCGg--------CCCGGGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 15373 | 0.66 | 0.104163 |
Target: 5'- -cGCc-GCCgGCGCCGCCaucgucgacgucggaGGGCCgCGCg -3' miRNA: 3'- cuCGacCGGgCGCGGCGG---------------CCCGG-GUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 24398 | 0.66 | 0.101369 |
Target: 5'- -uGCUGGCuaGaCGCCuucgucgaacgcuucGCCGGGUCCGa -3' miRNA: 3'- cuCGACCGggC-GCGG---------------CGGCCCGGGUg -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 22568 | 0.66 | 0.100271 |
Target: 5'- uGAGCuccuugucauUGaGCUCGCGCCGCCGcgaGGUgcugaCCACg -3' miRNA: 3'- -CUCG----------AC-CGGGCGCGGCGGC---CCG-----GGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 5268 | 0.66 | 0.100271 |
Target: 5'- cGAGCggguggagGGUCCgguccuuccaGgGCCGgaGGGCCCGCg -3' miRNA: 3'- -CUCGa-------CCGGG----------CgCGGCggCCCGGGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 25637 | 0.66 | 0.100271 |
Target: 5'- cGAGU--GCCCGcCGCCccuCUGGGCUCACu -3' miRNA: 3'- -CUCGacCGGGC-GCGGc--GGCCCGGGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 15977 | 0.66 | 0.097575 |
Target: 5'- -uGCUGGgCaCGCGCC-CCaGGCgCCGCu -3' miRNA: 3'- cuCGACCgG-GCGCGGcGGcCCG-GGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 25501 | 0.67 | 0.09599 |
Target: 5'- nAGCU-GCCCGCcugagaccggccgccGCCGCCGaGGCCgCGg -3' miRNA: 3'- cUCGAcCGGGCG---------------CGGCGGC-CCGG-GUg -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 17553 | 0.67 | 0.094947 |
Target: 5'- uGGCggUGGCa-GCGCCGCCc-GCCCGCa -3' miRNA: 3'- cUCG--ACCGggCGCGGCGGccCGGGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 7749 | 0.67 | 0.094688 |
Target: 5'- aGGCgGGCCUGCagggcguGCCGCaGGGCCUu- -3' miRNA: 3'- cUCGaCCGGGCG-------CGGCGgCCCGGGug -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 7252 | 0.67 | 0.092134 |
Target: 5'- uGGGCUGGuUCUGCuGCCGCCGcugcugcGGCCUu- -3' miRNA: 3'- -CUCGACC-GGGCG-CGGCGGC-------CCGGGug -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 23293 | 0.67 | 0.090882 |
Target: 5'- uGAGCUGcaCCCGUgggugccgcucggggGCCGCCGcGCCCGa -3' miRNA: 3'- -CUCGACc-GGGCG---------------CGGCGGCcCGGGUg -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 24653 | 0.67 | 0.084859 |
Target: 5'- cGGCUGcccuugcGCCgGCGCCGUCcccGGCCUGCg -3' miRNA: 3'- cUCGAC-------CGGgCGCGGCGGc--CCGGGUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 13214 | 0.67 | 0.079876 |
Target: 5'- -cGCUggGGCUCGCGCCGCuccagccuggccagCGGcGCCgACa -3' miRNA: 3'- cuCGA--CCGGGCGCGGCG--------------GCC-CGGgUG- -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 2052 | 0.68 | 0.076218 |
Target: 5'- cGGUgggGGCCgUGCGCaacaGCCGGGCCgCGg -3' miRNA: 3'- cUCGa--CCGG-GCGCGg---CGGCCCGG-GUg -5' |
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27419 | 3' | -68.7 | NC_005869.1 | + | 25803 | 0.68 | 0.076218 |
Target: 5'- -cGCUGGgacccCCCGC-CCGa-GGGCCCGCc -3' miRNA: 3'- cuCGACC-----GGGCGcGGCggCCCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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