Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27422 | 3' | -52 | NC_005869.1 | + | 2575 | 0.66 | 0.772566 |
Target: 5'- gGCucgGCGCUGCGCcCAGCg---CUUGCg -3' miRNA: 3'- gCG---UGUGGCGCGaGUUGaaugGAACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 15483 | 0.66 | 0.772566 |
Target: 5'- gCGC-CGCCGCGCgCGGCgccguUCUUcGCg -3' miRNA: 3'- -GCGuGUGGCGCGaGUUGaau--GGAA-CG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 15784 | 0.66 | 0.761795 |
Target: 5'- gGCACcgaCGCGCUCGGCcaugGCCaucucGCg -3' miRNA: 3'- gCGUGug-GCGCGAGUUGaa--UGGaa---CG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 15602 | 0.66 | 0.739818 |
Target: 5'- gGCGCuCCGCGC-CGGCcauCCUgggggUGCg -3' miRNA: 3'- gCGUGuGGCGCGaGUUGaauGGA-----ACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 24539 | 0.66 | 0.728639 |
Target: 5'- aGCgGCACCGCGCUgcGCgggggcGCCUucgagcUGCg -3' miRNA: 3'- gCG-UGUGGCGCGAguUGaa----UGGA------ACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 18035 | 0.66 | 0.728639 |
Target: 5'- gGCGCGCaCGCGCcgccccgucCAGCggcGCCUgggGCg -3' miRNA: 3'- gCGUGUG-GCGCGa--------GUUGaa-UGGAa--CG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 18198 | 0.66 | 0.728639 |
Target: 5'- cCGUACACCucggGCGC-CAAC--GCCU-GCa -3' miRNA: 3'- -GCGUGUGG----CGCGaGUUGaaUGGAaCG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 22903 | 0.67 | 0.717352 |
Target: 5'- aGCAgCugCGCGgUCAGCgcgcgggACCgggggUGCu -3' miRNA: 3'- gCGU-GugGCGCgAGUUGaa-----UGGa----ACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 26260 | 0.67 | 0.682975 |
Target: 5'- gCGCGCcaucgGCCGCGCcuUCAAg--GCCcUGCg -3' miRNA: 3'- -GCGUG-----UGGCGCG--AGUUgaaUGGaACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 17878 | 0.67 | 0.671388 |
Target: 5'- uGC-CGCCGgGCUCu-CUUGCCauaaGCa -3' miRNA: 3'- gCGuGUGGCgCGAGuuGAAUGGaa--CG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 22500 | 0.67 | 0.671388 |
Target: 5'- uGCACAaaGCGCUCGAUaUGCaauaaagGCu -3' miRNA: 3'- gCGUGUggCGCGAGUUGaAUGgaa----CG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 14339 | 0.67 | 0.671388 |
Target: 5'- gGCACcggGCUGCGCcaUCGACUUcACCUUc- -3' miRNA: 3'- gCGUG---UGGCGCG--AGUUGAA-UGGAAcg -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 9950 | 0.67 | 0.671388 |
Target: 5'- gGC-CACCacGCGCUCGGCcaggaugGCCU-GCg -3' miRNA: 3'- gCGuGUGG--CGCGAGUUGaa-----UGGAaCG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 9609 | 0.68 | 0.648105 |
Target: 5'- uCGCGCACC-UGCUCuuCggACCcgGCg -3' miRNA: 3'- -GCGUGUGGcGCGAGuuGaaUGGaaCG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 22949 | 0.68 | 0.643436 |
Target: 5'- aGCGCcCCGaccCGCUCAGCgcgaagggggugAUCUUGCa -3' miRNA: 3'- gCGUGuGGC---GCGAGUUGaa----------UGGAACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 17590 | 0.68 | 0.624754 |
Target: 5'- gCGgGCGCCcgGCGCUUGACg-GCCgUGCg -3' miRNA: 3'- -GCgUGUGG--CGCGAGUUGaaUGGaACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 13968 | 0.68 | 0.624754 |
Target: 5'- uGCAgucgacCACCGCGUUgAGCUgagagGCCUgaccgGCu -3' miRNA: 3'- gCGU------GUGGCGCGAgUUGAa----UGGAa----CG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 19028 | 0.68 | 0.613084 |
Target: 5'- aCGCgGCGCCGCGCgUCggUgacggUUACCcgcUGCa -3' miRNA: 3'- -GCG-UGUGGCGCG-AGuuG-----AAUGGa--ACG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 18549 | 0.68 | 0.613084 |
Target: 5'- gCGCgaagaacgGCGCCGCGCgCGGCggcGCCggGCu -3' miRNA: 3'- -GCG--------UGUGGCGCGaGUUGaa-UGGaaCG- -5' |
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27422 | 3' | -52 | NC_005869.1 | + | 24506 | 0.69 | 0.601432 |
Target: 5'- uGCAucauCAgUGCGCUCAGCcUGCCccucUGCa -3' miRNA: 3'- gCGU----GUgGCGCGAGUUGaAUGGa---ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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