Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27565 | 5' | -56.6 | NC_005882.1 | + | 12713 | 0.66 | 0.529347 |
Target: 5'- gGAGAcGCagcGCgCGGCGGCGCucGCCGGc- -3' miRNA: 3'- gCUUU-CGa--UG-GCCGCCGUG--CGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 26151 | 0.66 | 0.518493 |
Target: 5'- gCGAAGGC-GCCGGCaguGGCGacgaaGCCGu-- -3' miRNA: 3'- -GCUUUCGaUGGCCG---CCGUg----CGGCuau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 16893 | 0.66 | 0.517413 |
Target: 5'- gCGAccaaAAGCUgacgcaaGCCGGCGcGCguuucACGCCGGc- -3' miRNA: 3'- -GCU----UUCGA-------UGGCCGC-CG-----UGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 1626 | 0.66 | 0.507729 |
Target: 5'- uCGucGAGCUGCgCGGCGaCGC-CCGAUGc -3' miRNA: 3'- -GCu-UUCGAUG-GCCGCcGUGcGGCUAU- -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 19090 | 0.66 | 0.507729 |
Target: 5'- uGcAGGUUGCCGGCcG-ACGCCGAg- -3' miRNA: 3'- gCuUUCGAUGGCCGcCgUGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 18830 | 0.66 | 0.49706 |
Target: 5'- aCGAuuGC-GCCGGCGuGaUACGCgCGAUGc -3' miRNA: 3'- -GCUuuCGaUGGCCGC-C-GUGCG-GCUAU- -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 13086 | 0.66 | 0.49706 |
Target: 5'- uCGAugucgcGGCUGC-GGCGGCugGCGUCGAc- -3' miRNA: 3'- -GCUu-----UCGAUGgCCGCCG--UGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 20373 | 0.66 | 0.483344 |
Target: 5'- uCGGucGCUGucuggaCGGCGGCuuguuccagggggaACGCCGAUu -3' miRNA: 3'- -GCUuuCGAUg-----GCCGCCG--------------UGCGGCUAu -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 31961 | 0.66 | 0.476035 |
Target: 5'- cCGAcGAGCgu-CGGCGGC-CGCUGAc- -3' miRNA: 3'- -GCU-UUCGaugGCCGCCGuGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 28033 | 0.66 | 0.474995 |
Target: 5'- cCGGAAGCcgaGCUGGCGaagcugcGCGCGgCGGUGa -3' miRNA: 3'- -GCUUUCGa--UGGCCGC-------CGUGCgGCUAU- -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 31448 | 0.67 | 0.465689 |
Target: 5'- uCGcgGGCUgcGCCGGCaGCcgcgucACGCCGAc- -3' miRNA: 3'- -GCuuUCGA--UGGCCGcCG------UGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 29867 | 0.67 | 0.455461 |
Target: 5'- -cGAAGCUgGCCGcCGGUGCGUCGAUc -3' miRNA: 3'- gcUUUCGA-UGGCcGCCGUGCGGCUAu -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 32601 | 0.67 | 0.445355 |
Target: 5'- uCGAGGGC-AUCGGUGGCGCcuacuaCGAUGg -3' miRNA: 3'- -GCUUUCGaUGGCCGCCGUGcg----GCUAU- -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 31070 | 0.67 | 0.445355 |
Target: 5'- ---cGGCUcccACCGGCGGauCGCGUCGAc- -3' miRNA: 3'- gcuuUCGA---UGGCCGCC--GUGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 12790 | 0.67 | 0.435376 |
Target: 5'- gCGAAcaGGUcGCCGGCGaGCGcCGCCGc-- -3' miRNA: 3'- -GCUU--UCGaUGGCCGC-CGU-GCGGCuau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 16074 | 0.68 | 0.396798 |
Target: 5'- aCGGccGcCUugCGGCGGCGCacgcacccGCCGAc- -3' miRNA: 3'- -GCUuuC-GAugGCCGCCGUG--------CGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 12944 | 0.68 | 0.378351 |
Target: 5'- -cGGAGCUguugcGCCGGCc-CGCGCCGAUGa -3' miRNA: 3'- gcUUUCGA-----UGGCCGccGUGCGGCUAU- -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 20947 | 0.68 | 0.369346 |
Target: 5'- aGAucAGGUaGCCGGCGucCGCGCCGAg- -3' miRNA: 3'- gCU--UUCGaUGGCCGCc-GUGCGGCUau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 4740 | 0.69 | 0.350919 |
Target: 5'- aCGAucgcgucGAGCUGCUcuuGCGGUACGCCGu-- -3' miRNA: 3'- -GCU-------UUCGAUGGc--CGCCGUGCGGCuau -5' |
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27565 | 5' | -56.6 | NC_005882.1 | + | 3453 | 0.69 | 0.343223 |
Target: 5'- gCGAAGucuGCUGCCuGaCGGCACGCgGGUu -3' miRNA: 3'- -GCUUU---CGAUGGcC-GCCGUGCGgCUAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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