Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27569 | 3' | -50.5 | NC_005882.1 | + | 7324 | 0.66 | 0.874209 |
Target: 5'- gGCGAGCUgauUCGCugCGUCGAcCAUUGCg -3' miRNA: 3'- -CGUUUGGag-AGCG--GCAGCUuGUAGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 25273 | 0.66 | 0.857092 |
Target: 5'- cGCGAuuGCCggcgucgugCUCGCCGUCuGGCuguUCAUc -3' miRNA: 3'- -CGUU--UGGa--------GAGCGGCAGcUUGu--AGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 33140 | 0.66 | 0.857092 |
Target: 5'- gGCAAGCCU--UGCCG-CGAGguccgacuuCGUCGCg -3' miRNA: 3'- -CGUUUGGAgaGCGGCaGCUU---------GUAGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 25396 | 0.66 | 0.856208 |
Target: 5'- cGCugaauGGCCUCggccgcgUCGCCGaCGAGCugcgCGCg -3' miRNA: 3'- -CGu----UUGGAG-------AGCGGCaGCUUGua--GUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 16811 | 0.66 | 0.838916 |
Target: 5'- aCGAccGCCUUgUCGCCGUCGcGAUGUCGu -3' miRNA: 3'- cGUU--UGGAG-AGCGGCAGC-UUGUAGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 30613 | 0.66 | 0.838916 |
Target: 5'- cGUGAACUcggUCUCcgGCaCGUCGAcCGUCACc -3' miRNA: 3'- -CGUUUGG---AGAG--CG-GCAGCUuGUAGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 28639 | 0.66 | 0.838916 |
Target: 5'- cCAAGCCgccuugCGCCGaUCGGACcgCAUc -3' miRNA: 3'- cGUUUGGaga---GCGGC-AGCUUGuaGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 2885 | 0.66 | 0.838916 |
Target: 5'- aCAAGCUUCcccaUCGCCcgaugaucgaaGUCGAAC-UCGCg -3' miRNA: 3'- cGUUUGGAG----AGCGG-----------CAGCUUGuAGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 362 | 0.67 | 0.829455 |
Target: 5'- uCAGACUUCUCcgcaGCCGUCGAuguaauGCcgCAg -3' miRNA: 3'- cGUUUGGAGAG----CGGCAGCU------UGuaGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 2260 | 0.67 | 0.819761 |
Target: 5'- uGCAGGCCg--CGaaGUCGAugAUCGa -3' miRNA: 3'- -CGUUUGGagaGCggCAGCUugUAGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 12791 | 0.67 | 0.819761 |
Target: 5'- gGCGAACaggUCGCCGgCGAGCGcCGCc -3' miRNA: 3'- -CGUUUGgagAGCGGCaGCUUGUaGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 5921 | 0.67 | 0.809844 |
Target: 5'- cGCAGGCCgUUCGCa--CGGGCAUCGg -3' miRNA: 3'- -CGUUUGGaGAGCGgcaGCUUGUAGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 23261 | 0.67 | 0.799718 |
Target: 5'- cGCGAGCgUCgUGCCGaUCGGcGCAUCGa -3' miRNA: 3'- -CGUUUGgAGaGCGGC-AGCU-UGUAGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 5668 | 0.67 | 0.799718 |
Target: 5'- ---cGCCgugUUCGCCGauccggcgUCGAGCAUCAg -3' miRNA: 3'- cguuUGGa--GAGCGGC--------AGCUUGUAGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 34482 | 0.68 | 0.778885 |
Target: 5'- aGCGGGCCgucggCUCgGUCGUCGGAUcgaugCGCa -3' miRNA: 3'- -CGUUUGGa----GAG-CGGCAGCUUGua---GUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 12119 | 0.68 | 0.768205 |
Target: 5'- uCAuGCC-CUCGaCCGUCGAcuGgAUCGCg -3' miRNA: 3'- cGUuUGGaGAGC-GGCAGCU--UgUAGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 28647 | 0.68 | 0.757367 |
Target: 5'- cGCGGGCCagcUCUCGaCGgCGAGCAgCGCg -3' miRNA: 3'- -CGUUUGG---AGAGCgGCaGCUUGUaGUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 12662 | 0.68 | 0.746386 |
Target: 5'- cGCAGACggCgcgcgcgaUGCCGUCGAACAagCGCg -3' miRNA: 3'- -CGUUUGgaGa-------GCGGCAGCUUGUa-GUG- -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 15597 | 0.68 | 0.735273 |
Target: 5'- cGCGGACCUgcgCGCCGUacaGGuCAUCAa -3' miRNA: 3'- -CGUUUGGAga-GCGGCAg--CUuGUAGUg -5' |
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27569 | 3' | -50.5 | NC_005882.1 | + | 17650 | 0.69 | 0.70129 |
Target: 5'- gGCGGGCCgCUaCGCCGagcaaauggCGAACGUCGa -3' miRNA: 3'- -CGUUUGGaGA-GCGGCa--------GCUUGUAGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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