Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27575 | 3' | -55.9 | NC_005882.1 | + | 16333 | 0.67 | 0.452678 |
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Target: 5'- -cCuGGUGCC-GCGCCAggaggCGACGuAUCu -3' miRNA: 3'- uaGcCCACGGuUGCGGUa----GCUGC-UAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 18188 | 0.67 | 0.452678 |
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Target: 5'- -gCGcGUGCgCAGCGCCGagUCGuCGAUCu -3' miRNA: 3'- uaGCcCACG-GUUGCGGU--AGCuGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 23442 | 0.67 | 0.483773 |
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Target: 5'- -aCGGGcGUCGAgcggcCGCUGUCGGCGAUg -3' miRNA: 3'- uaGCCCaCGGUU-----GCGGUAGCUGCUAg -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 16008 | 0.66 | 0.537716 |
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Target: 5'- -gCGGGUGCguGCGCCGccgcaaGGCGGc- -3' miRNA: 3'- uaGCCCACGguUGCGGUag----CUGCUag -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 32067 | 0.66 | 0.537716 |
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Target: 5'- gAUCGcGUaGUCGAcCGgCGUCGGCGAUCg -3' miRNA: 3'- -UAGCcCA-CGGUU-GCgGUAGCUGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 16160 | 0.66 | 0.548765 |
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Target: 5'- gGUCGaGGU-CC-GCGaagaCGUCGGCGAUCg -3' miRNA: 3'- -UAGC-CCAcGGuUGCg---GUAGCUGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 23830 | 0.66 | 0.548765 |
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Target: 5'- -gCGGGccggaucgaaccaUGCCuugaagccgagcagcGCGCCGUCGcCGAUCa -3' miRNA: 3'- uaGCCC-------------ACGGu--------------UGCGGUAGCuGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 31939 | 0.66 | 0.569945 |
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Target: 5'- -gCGGGUagaccuggacgcuGCCuggcugAACGCCGUUGACGGa- -3' miRNA: 3'- uaGCCCA-------------CGG------UUGCGGUAGCUGCUag -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 13124 | 0.66 | 0.571066 |
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Target: 5'- ---uGGUGUCGAUGCUcgCGACGuUCa -3' miRNA: 3'- uagcCCACGGUUGCGGuaGCUGCuAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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