Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27575 | 3' | -55.9 | NC_005882.1 | + | 21422 | 0.7 | 0.340398 |
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Target: 5'- -aCGGcGcaCCGGCGCCGaCGACGAUCa -3' miRNA: 3'- uaGCC-CacGGUUGCGGUaGCUGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 24802 | 0.7 | 0.315666 |
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Target: 5'- gGUCGaGUucGCCGAUGUCuUCGACGGUCa -3' miRNA: 3'- -UAGCcCA--CGGUUGCGGuAGCUGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 16880 | 0.7 | 0.307729 |
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Target: 5'- -aCGGGUcGaUCGGCGCCGUCGAaguucaGGUCg -3' miRNA: 3'- uaGCCCA-C-GGUUGCGGUAGCUg-----CUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 22970 | 0.71 | 0.299946 |
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Target: 5'- -cCGGGUGauGACGCCGUUGAUcaGGUCg -3' miRNA: 3'- uaGCCCACggUUGCGGUAGCUG--CUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 12020 | 0.71 | 0.299946 |
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Target: 5'- -gCGcGGUacGCCAGCGCC-UCGGCGggCa -3' miRNA: 3'- uaGC-CCA--CGGUUGCGGuAGCUGCuaG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 27539 | 0.71 | 0.29156 |
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Target: 5'- --aGGGcGCCGcaaucggcaucggGCGCuCGUCGGCGGUCg -3' miRNA: 3'- uagCCCaCGGU-------------UGCG-GUAGCUGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 27207 | 0.71 | 0.277511 |
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Target: 5'- gAUCGGGauUGCCAucacCGCgAUCG-CGGUCg -3' miRNA: 3'- -UAGCCC--ACGGUu---GCGgUAGCuGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 12536 | 0.73 | 0.218232 |
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Target: 5'- uGUCGcGGcggcgGCCGGCGCUG-CGGCGAUCg -3' miRNA: 3'- -UAGC-CCa----CGGUUGCGGUaGCUGCUAG- -5' |
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| 27575 | 3' | -55.9 | NC_005882.1 | + | 9475 | 1.08 | 0.000545 |
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Target: 5'- cAUCGGGUGCCAACGCCAUCGACGAUCg -3' miRNA: 3'- -UAGCCCACGGUUGCGGUAGCUGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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