Results 1 - 20 of 79 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27996 | 5' | -56.7 | NC_005887.1 | + | 21547 | 0.66 | 0.55311 |
Target: 5'- aCGA--CGCUGCAGG-CGAcCGGCGCa -3' miRNA: 3'- aGCUccGCGGCGUCCaGUUuGUCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 18396 | 0.66 | 0.55311 |
Target: 5'- cCGAGGuCGUCGguGcGUgcgCAGACGGcCGCg -3' miRNA: 3'- aGCUCC-GCGGCguC-CA---GUUUGUC-GUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 34935 | 0.66 | 0.55311 |
Target: 5'- aCGAGGCgGCgGCGGGUgcGACcguGGCGa -3' miRNA: 3'- aGCUCCG-CGgCGUCCAguUUG---UCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 6043 | 0.66 | 0.542169 |
Target: 5'- cCGGguGGUGCUGguGG-CAGcggcGCGGCACu -3' miRNA: 3'- aGCU--CCGCGGCguCCaGUU----UGUCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 2488 | 0.66 | 0.542169 |
Target: 5'- gCGAGGCGCUcaGCGgugagcGGUCGauugaugugcGACGGCGg -3' miRNA: 3'- aGCUCCGCGG--CGU------CCAGU----------UUGUCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 10612 | 0.66 | 0.532384 |
Target: 5'- aCGGGGCGCagcgaagcguuugcaGCAGG-CAGaacGCcGCACg -3' miRNA: 3'- aGCUCCGCGg--------------CGUCCaGUU---UGuCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 17284 | 0.66 | 0.531301 |
Target: 5'- gUCGGgcGGCGCCGCGcgcgcaaacgccGGUU---CGGCGCa -3' miRNA: 3'- -AGCU--CCGCGGCGU------------CCAGuuuGUCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 12708 | 0.66 | 0.531301 |
Target: 5'- aCGc-GCGCaCGCuGGUCGcGCAGCAg -3' miRNA: 3'- aGCucCGCG-GCGuCCAGUuUGUCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 19181 | 0.66 | 0.520513 |
Target: 5'- cCGuGaGCGgCGCGGGcguggagaCAGGCGGCGCg -3' miRNA: 3'- aGCuC-CGCgGCGUCCa-------GUUUGUCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 29847 | 0.66 | 0.520513 |
Target: 5'- uUUGAGGaUGUCGCAGG-CGAugaacacguccuGCGGCAg -3' miRNA: 3'- -AGCUCC-GCGGCGUCCaGUU------------UGUCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 1388 | 0.66 | 0.519439 |
Target: 5'- aUC-AGGC-CCGCGccgaagcGGUCGAGCAuGCGCu -3' miRNA: 3'- -AGcUCCGcGGCGU-------CCAGUUUGU-CGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 16858 | 0.66 | 0.509812 |
Target: 5'- gCGAGGUGuaCCGCGGcG-CGGACGGCuCg -3' miRNA: 3'- aGCUCCGC--GGCGUC-CaGUUUGUCGuG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 34983 | 0.66 | 0.509812 |
Target: 5'- aCGcGGCGuuGUcgucGGUCu-GCAGCGCg -3' miRNA: 3'- aGCuCCGCggCGu---CCAGuuUGUCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 23334 | 0.67 | 0.503435 |
Target: 5'- uUCGAagaggugacagucauGGCGCgGCcuuuGGUUAGACuGCGCc -3' miRNA: 3'- -AGCU---------------CCGCGgCGu---CCAGUUUGuCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 32796 | 0.67 | 0.488695 |
Target: 5'- aUCGGcGcGCGCCGCuucacGGgcaCGAACAGCGg -3' miRNA: 3'- -AGCU-C-CGCGGCGu----CCa--GUUUGUCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 34845 | 0.67 | 0.488695 |
Target: 5'- cUCGGGGUcguGCCGCcgaGGGUguaguucaagcgCAggUAGCGCu -3' miRNA: 3'- -AGCUCCG---CGGCG---UCCA------------GUuuGUCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 7217 | 0.67 | 0.488695 |
Target: 5'- gCGcAGGCGCUGuCGGcGUCGGGC-GCAUc -3' miRNA: 3'- aGC-UCCGCGGC-GUC-CAGUUUGuCGUG- -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 20121 | 0.67 | 0.48244 |
Target: 5'- uUCGAGGCguacaacuacuaucaGCUGUGGGaugcgcaaacgUCAAGCGGCGg -3' miRNA: 3'- -AGCUCCG---------------CGGCGUCC-----------AGUUUGUCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 5822 | 0.67 | 0.478291 |
Target: 5'- aUCGAGuCGgCGCGGGUCGcgAAgGGCGa -3' miRNA: 3'- -AGCUCcGCgGCGUCCAGU--UUgUCGUg -5' |
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27996 | 5' | -56.7 | NC_005887.1 | + | 27398 | 0.67 | 0.478291 |
Target: 5'- gUCGAGuuGCgGCGcGUCGuacGGCAGCGCg -3' miRNA: 3'- -AGCUCcgCGgCGUcCAGU---UUGUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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