Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27997 | 3' | -53.7 | NC_005887.1 | + | 26334 | 0.66 | 0.738148 |
Target: 5'- cCGUGG-CCGG--CGCUggCCGGguGCg -3' miRNA: 3'- -GUACUaGGCCuuGCGGaaGGUCguCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 17873 | 0.66 | 0.735975 |
Target: 5'- aCGUGGgcCCGGAAccugucCGCCgcgacgaugaagUUCGGCGGCg -3' miRNA: 3'- -GUACUa-GGCCUU------GCGGa-----------AGGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 32419 | 0.66 | 0.72724 |
Target: 5'- aCGUGG-CCGGcGGCGauguggCCGGCGGCg -3' miRNA: 3'- -GUACUaGGCC-UUGCggaa--GGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 2851 | 0.66 | 0.72724 |
Target: 5'- gCGUGAacuUCCaGuucgucaGCCUggCCAGCGGCa -3' miRNA: 3'- -GUACU---AGGcCuug----CGGAa-GGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 40548 | 0.66 | 0.716231 |
Target: 5'- --aGGUCggCGGcACGuCCUUCgGGUAGCg -3' miRNA: 3'- guaCUAG--GCCuUGC-GGAAGgUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 40838 | 0.66 | 0.705134 |
Target: 5'- --aGGUCCGGGuACGUgUUCCgcacGGCcGCg -3' miRNA: 3'- guaCUAGGCCU-UGCGgAAGG----UCGuCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 30135 | 0.66 | 0.69396 |
Target: 5'- gCGUGAaaUCCGGcGCGCCcggc-GCGGCg -3' miRNA: 3'- -GUACU--AGGCCuUGCGGaagguCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 2169 | 0.67 | 0.682722 |
Target: 5'- gCAUGGacUUCGGccuGACGCCggCCgcggugaucgGGCAGCg -3' miRNA: 3'- -GUACU--AGGCC---UUGCGGaaGG----------UCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 2529 | 0.67 | 0.660104 |
Target: 5'- --cGGcCCGucGCGCUUuaUCCAGCAGUg -3' miRNA: 3'- guaCUaGGCcuUGCGGA--AGGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 24949 | 0.67 | 0.660104 |
Target: 5'- gCGUGcgCgCGGGcCGCCUUgCGcGCGGCc -3' miRNA: 3'- -GUACuaG-GCCUuGCGGAAgGU-CGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 41459 | 0.67 | 0.660104 |
Target: 5'- --cGAagCCGaGGcCGCCUgacucgUCCGGCGGCg -3' miRNA: 3'- guaCUa-GGC-CUuGCGGA------AGGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 26573 | 0.67 | 0.648747 |
Target: 5'- --cGAUCUGcuucuGCGCCUUCgCGGCcGCg -3' miRNA: 3'- guaCUAGGCcu---UGCGGAAG-GUCGuCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 31941 | 0.67 | 0.637374 |
Target: 5'- --cGA-CCGuAACGCCUUCgAGguGCc -3' miRNA: 3'- guaCUaGGCcUUGCGGAAGgUCguCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 28731 | 0.67 | 0.637374 |
Target: 5'- aGUGcggCCGccgcGCGCCgcgcugCCAGCAGCg -3' miRNA: 3'- gUACua-GGCcu--UGCGGaa----GGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 20825 | 0.67 | 0.635099 |
Target: 5'- uCGUGAauuucccggcagCCGGcgucguuGCGCCgauugCCGGCGGCg -3' miRNA: 3'- -GUACUa-----------GGCCu------UGCGGaa---GGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 1041 | 0.67 | 0.625996 |
Target: 5'- cCGUGAUgCGGugUuCCUUgcCCGGCGGCa -3' miRNA: 3'- -GUACUAgGCCuuGcGGAA--GGUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 34671 | 0.67 | 0.625996 |
Target: 5'- ----uUCCGGAucCGCCacguugUCgAGCAGCa -3' miRNA: 3'- guacuAGGCCUu-GCGGa-----AGgUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 22355 | 0.68 | 0.603268 |
Target: 5'- gCAUGAugaugcagucUCCGGucgcAUGCgCUUCCAGCAa- -3' miRNA: 3'- -GUACU----------AGGCCu---UGCG-GAAGGUCGUcg -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 36973 | 0.68 | 0.591938 |
Target: 5'- --cGcgCUGGcuuCGCCUUCgAGCAGUc -3' miRNA: 3'- guaCuaGGCCuu-GCGGAAGgUCGUCG- -5' |
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27997 | 3' | -53.7 | NC_005887.1 | + | 18104 | 0.68 | 0.580645 |
Target: 5'- cCGUGGcugcugCCGGcACGUgg-CCAGCAGCg -3' miRNA: 3'- -GUACUa-----GGCCuUGCGgaaGGUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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