Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28001 | 3' | -54.7 | NC_005887.1 | + | 10989 | 0.72 | 0.276132 |
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Target: 5'- cGCUCGucgagGAUGGCguggUGCucgGGUGCGGCGGu -3' miRNA: 3'- -CGAGC-----UUGCCGa---ACG---UCACGUCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 7014 | 0.72 | 0.312897 |
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Target: 5'- aGCUCGuggccggccgccGCGGCgcgcgGCAGUGgAGCGAu -3' miRNA: 3'- -CGAGCu-----------UGCCGaa---CGUCACgUCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 1927 | 0.68 | 0.516364 |
|
Target: 5'- aGC-CGGGCGGCgucaUGC-GUGaCGGCGAu -3' miRNA: 3'- -CGaGCUUGCCGa---ACGuCAC-GUCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 1497 | 0.66 | 0.594829 |
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Target: 5'- cGCUCGcGCGGCgcgGCcGUGCGGa--- -3' miRNA: 3'- -CGAGCuUGCCGaa-CGuCACGUCgcuu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 443 | 0.68 | 0.516364 |
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Target: 5'- cGCUCGAACccucuGGCgcgUGUGGUGCGGgcaUGAGc -3' miRNA: 3'- -CGAGCUUG-----CCGa--ACGUCACGUC---GCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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