Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28001 | 3' | -54.7 | NC_005887.1 | + | 26881 | 0.69 | 0.462936 |
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Target: 5'- aGCUCGAACGcGuCUUggugcGCGGUcaucGCGGCGAc -3' miRNA: 3'- -CGAGCUUGC-C-GAA-----CGUCA----CGUCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 15393 | 0.69 | 0.467106 |
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Target: 5'- cGC-CGAACGGCcUGCAGgaucuguggggccaGCAGUGGu -3' miRNA: 3'- -CGaGCUUGCCGaACGUCa-------------CGUCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 15762 | 0.66 | 0.603962 |
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Target: 5'- aGCUcgCGAACGGCgccgucuacaaGCAGgaggacgacgGCAGCGAc -3' miRNA: 3'- -CGA--GCUUGCCGaa---------CGUCa---------CGUCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 40736 | 0.66 | 0.64061 |
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Target: 5'- aGCUCGAAUGaCagGUAGUGCguuGGCGGu -3' miRNA: 3'- -CGAGCUUGCcGaaCGUCACG---UCGCUu -5' |
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| 28001 | 3' | -54.7 | NC_005887.1 | + | 40232 | 1.08 | 0.000743 |
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Target: 5'- aGCUCGAACGGCUUGCAGUGCAGCGAAu -3' miRNA: 3'- -CGAGCUUGCCGAACGUCACGUCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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