Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28001 | 3' | -54.7 | NC_005887.1 | + | 25500 | 0.69 | 0.442385 |
Target: 5'- gGCUCGGACgauGGCgcgGCGGgugGCGGCa-- -3' miRNA: 3'- -CGAGCUUG---CCGaa-CGUCa--CGUCGcuu -5' |
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28001 | 3' | -54.7 | NC_005887.1 | + | 41504 | 0.69 | 0.462936 |
Target: 5'- gGCUCGAACGGCaaGUcgacGGUGCucGGCa-- -3' miRNA: 3'- -CGAGCUUGCCGaaCG----UCACG--UCGcuu -5' |
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28001 | 3' | -54.7 | NC_005887.1 | + | 1927 | 0.68 | 0.516364 |
Target: 5'- aGC-CGGGCGGCgucaUGC-GUGaCGGCGAu -3' miRNA: 3'- -CGaGCUUGCCGa---ACGuCAC-GUCGCUu -5' |
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28001 | 3' | -54.7 | NC_005887.1 | + | 443 | 0.68 | 0.516364 |
Target: 5'- cGCUCGAACccucuGGCgcgUGUGGUGCGGgcaUGAGc -3' miRNA: 3'- -CGAGCUUG-----CCGa--ACGUCACGUC---GCUU- -5' |
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28001 | 3' | -54.7 | NC_005887.1 | + | 11300 | 0.74 | 0.235131 |
Target: 5'- uGCUCGcAGCGGCcgGCucgGCAGCGAGc -3' miRNA: 3'- -CGAGC-UUGCCGaaCGucaCGUCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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