Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28024 | 5' | -56.3 | NC_005887.1 | + | 3400 | 0.66 | 0.566062 |
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Target: 5'- uUCGCCGCGcgCuACCgcugGCagGgCGGCu -3' miRNA: 3'- uAGCGGCGCaaG-UGGa---CGaaCaGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 28305 | 0.66 | 0.566062 |
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Target: 5'- cGUCGUgGCGacUugUUGCUggaagccuguUGUCGGCg -3' miRNA: 3'- -UAGCGgCGCaaGugGACGA----------ACAGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 38011 | 0.66 | 0.566062 |
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Target: 5'- -gCGCCgGCGUcgaUCAguuCCUGCggcaUGUcCGGCa -3' miRNA: 3'- uaGCGG-CGCA---AGU---GGACGa---ACA-GCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 37743 | 0.66 | 0.566062 |
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Target: 5'- -gCGCCGCGgcgagCAUCUGUUgcaucUGcgCGGCc -3' miRNA: 3'- uaGCGGCGCaa---GUGGACGA-----ACa-GCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 23780 | 0.66 | 0.577126 |
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Target: 5'- -aCGCCGCGacgagcaugUCGCCcGCgaaGcCGGCg -3' miRNA: 3'- uaGCGGCGCa--------AGUGGaCGaa-CaGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 4656 | 0.66 | 0.577126 |
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Target: 5'- gAUCGCCcccgGCG-UCGCUUGCaUGcggggCGGCu -3' miRNA: 3'- -UAGCGG----CGCaAGUGGACGaACa----GCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 34632 | 0.66 | 0.587125 |
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Target: 5'- uUCGCCGUucgcgucgacgagGcgCGCCgGCUUGcCGGUu -3' miRNA: 3'- uAGCGGCG-------------CaaGUGGaCGAACaGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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