Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28024 | 5' | -56.3 | NC_005887.1 | + | 22991 | 0.66 | 0.544108 |
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Target: 5'- cGUCGCCGCacgcgUCugCUGCgcgagcaugGUCaGCu -3' miRNA: 3'- -UAGCGGCGca---AGugGACGaa-------CAGcCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 8397 | 0.66 | 0.543018 |
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Target: 5'- -aCGCCGCacgcgacGUgcUCGCCgaGCgccGUCGGCa -3' miRNA: 3'- uaGCGGCG-------CA--AGUGGa-CGaa-CAGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 29373 | 0.66 | 0.533235 |
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Target: 5'- -gUGCCGCGggUCGCC-GCU--UCGGUc -3' miRNA: 3'- uaGCGGCGCa-AGUGGaCGAacAGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 25115 | 0.67 | 0.522439 |
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Target: 5'- -aCGCCGcCGUacgcgaUCGugucccacacgcCCUGCUUGcgCGGCg -3' miRNA: 3'- uaGCGGC-GCA------AGU------------GGACGAACa-GCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 14542 | 0.67 | 0.499 |
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Target: 5'- --aGCCGCuaucuauucccUCGCCUGC--GUCGGCg -3' miRNA: 3'- uagCGGCGca---------AGUGGACGaaCAGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 21228 | 0.67 | 0.490592 |
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Target: 5'- uUCGUgGCGUUggcgagCACCgUGUUUG-CGGCg -3' miRNA: 3'- uAGCGgCGCAA------GUGG-ACGAACaGCCG- -5' |
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| 28024 | 5' | -56.3 | NC_005887.1 | + | 23780 | 0.66 | 0.577126 |
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Target: 5'- -aCGCCGCGacgagcaugUCGCCcGCgaaGcCGGCg -3' miRNA: 3'- uaGCGGCGCa--------AGUGGaCGaa-CaGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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