Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28048 | 5' | -56.7 | NC_005887.1 | + | 36238 | 0.67 | 0.483959 |
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Target: 5'- aGGCACCACUucGCCAgcuCGUGCGCggCCg -3' miRNA: 3'- cUCGUGGUGGuaCGGU---GUAUGCG--GGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 40856 | 0.66 | 0.494481 |
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Target: 5'- -cGCACgGCCGcGCCGCGcgaGCGCCg- -3' miRNA: 3'- cuCGUGgUGGUaCGGUGUa--UGCGGga -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 30923 | 0.66 | 0.494481 |
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Target: 5'- cGGCAUCGCCuuGUGCCAgc-GCGCUCg -3' miRNA: 3'- cUCGUGGUGG--UACGGUguaUGCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 23159 | 0.66 | 0.515828 |
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Target: 5'- -cGCGCCGCC-UGUCucc-ACGCCCg -3' miRNA: 3'- cuCGUGGUGGuACGGuguaUGCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 19259 | 0.66 | 0.515828 |
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Target: 5'- -uGUACCGCUugGUGCCGCcgACGCa-- -3' miRNA: 3'- cuCGUGGUGG--UACGGUGuaUGCGgga -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 14021 | 0.66 | 0.515828 |
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Target: 5'- -cGCaACCGCCGgcgcGCCGCcgGCGCagCCUg -3' miRNA: 3'- cuCG-UGGUGGUa---CGGUGuaUGCG--GGA- -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 12933 | 0.66 | 0.526641 |
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Target: 5'- cGAGCGCguCCcUGCCGCAgcaaGCCg- -3' miRNA: 3'- -CUCGUGguGGuACGGUGUaug-CGGga -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 19076 | 0.66 | 0.526641 |
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Target: 5'- cGGGCugCGgCAaccGCCACGagcUGCGCCUUu -3' miRNA: 3'- -CUCGugGUgGUa--CGGUGU---AUGCGGGA- -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 11220 | 0.66 | 0.537538 |
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Target: 5'- -cGCACgGCC-UGCCcgGCAagcuCGCCCUg -3' miRNA: 3'- cuCGUGgUGGuACGG--UGUau--GCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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