Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28174 | 5' | -56.9 | NC_005887.1 | + | 14094 | 0.69 | 0.376183 |
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Target: 5'- -aUGCUCGUCAGUgGUgaagCGCCGcUCGc -3' miRNA: 3'- gaACGGGCGGUCGgUAa---GCGGCaAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 14014 | 0.69 | 0.394251 |
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Target: 5'- -aUGCCgCGCCGGCgCAgaCGCCGa--- -3' miRNA: 3'- gaACGG-GCGGUCG-GUaaGCGGCaagc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 11870 | 0.71 | 0.272856 |
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Target: 5'- --cGCUCGgCGGUCuUUCGUCGUUCGg -3' miRNA: 3'- gaaCGGGCgGUCGGuAAGCGGCAAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 9927 | 0.66 | 0.534754 |
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Target: 5'- --cG-CCGCCGGCCAcaUCGCCGccggccacgUCGa -3' miRNA: 3'- gaaCgGGCGGUCGGUa-AGCGGCa--------AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 8978 | 0.72 | 0.252501 |
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Target: 5'- -cUGCCCGCCGGCgCGUgcacaucCGCUGcagUCGg -3' miRNA: 3'- gaACGGGCGGUCG-GUAa------GCGGCa--AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 8106 | 0.66 | 0.545546 |
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Target: 5'- --aGCUCGCCGcgcucGCCGaggcgcCGCUGUUCGg -3' miRNA: 3'- gaaCGGGCGGU-----CGGUaa----GCGGCAAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 7292 | 0.7 | 0.32542 |
|
Target: 5'- --cGCCCGCUgugGGUCGUcgugUCGCCGUccgUCGc -3' miRNA: 3'- gaaCGGGCGG---UCGGUA----AGCGGCA---AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 4617 | 0.66 | 0.567329 |
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Target: 5'- -aUGCUCGCCGcggcGCCGguggcCGCCGgcgCGa -3' miRNA: 3'- gaACGGGCGGU----CGGUaa---GCGGCaa-GC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 998 | 0.66 | 0.513406 |
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Target: 5'- aUUGCCgGCC-GCCGUgcgcgCGCUGUa-- -3' miRNA: 3'- gAACGGgCGGuCGGUAa----GCGGCAagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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