Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28174 | 5' | -56.9 | NC_005887.1 | + | 7292 | 0.7 | 0.32542 |
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Target: 5'- --cGCCCGCUgugGGUCGUcgugUCGCCGUccgUCGc -3' miRNA: 3'- gaaCGGGCGG---UCGGUA----AGCGGCA---AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 29700 | 0.7 | 0.309669 |
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Target: 5'- -cUGCaCGCgCAGCCAcUCGCCGUcgCGu -3' miRNA: 3'- gaACGgGCG-GUCGGUaAGCGGCAa-GC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 23888 | 0.71 | 0.279927 |
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Target: 5'- cCUUGCCCGCgAGCagguucUUCGCCugcgUCGa -3' miRNA: 3'- -GAACGGGCGgUCGgu----AAGCGGca--AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 11870 | 0.71 | 0.272856 |
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Target: 5'- --cGCUCGgCGGUCuUUCGUCGUUCGg -3' miRNA: 3'- gaaCGGGCgGUCGGuAAGCGGCAAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 39715 | 0.71 | 0.272856 |
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Target: 5'- -gUGCCCGCC-GCCAccucauccUCGCgCGUUCu -3' miRNA: 3'- gaACGGGCGGuCGGUa-------AGCG-GCAAGc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 8978 | 0.72 | 0.252501 |
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Target: 5'- -cUGCCCGCCGGCgCGUgcacaucCGCUGcagUCGg -3' miRNA: 3'- gaACGGGCGGUCG-GUAa------GCGGCa--AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 20195 | 0.72 | 0.233413 |
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Target: 5'- uUUGCgCGUCAGCCcUUCGCCGa--- -3' miRNA: 3'- gAACGgGCGGUCGGuAAGCGGCaagc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 30205 | 0.74 | 0.16888 |
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Target: 5'- --cGCCgGCCAGCCA--CGCCGcgUCGc -3' miRNA: 3'- gaaCGGgCGGUCGGUaaGCGGCa-AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 34616 | 1.08 | 0.000577 |
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Target: 5'- cCUUGCCCGCCAGCCAUUCGCCGUUCGc -3' miRNA: 3'- -GAACGGGCGGUCGGUAAGCGGCAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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