Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28207 | 3' | -52.9 | NC_005891.1 | + | 21747 | 0.93 | 0.017262 |
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Target: 5'- aGGGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaugaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 24638 | 0.94 | 0.014808 |
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Target: 5'- aGGGUGUCGACGUCGACACCC-ACa-- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaUGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 29339 | 0.97 | 0.00889 |
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Target: 5'- aGGGUGUCGACGUCGACACCCUcuuuuuacuggagagACUUAa -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGA---------------UGAAU- -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 29279 | 0.98 | 0.008007 |
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Target: 5'- aGGGUGUCGACGUCGACACCCUAg--- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUgaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 24578 | 1 | 0.005532 |
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Target: 5'- uGGGUGUCGACGUCGACACCCUAUUc- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 21807 | 1.06 | 0.002261 |
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Target: 5'- uGGGUGUCGACGUCGACACCCUACUg- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 34767 | 1.09 | 0.001219 |
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Target: 5'- uGGGUGUCGACGUCGACACCCUACUUAa -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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