Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28466 | 3' | -55.8 | NC_005946.1 | + | 42858 | 0.66 | 0.845111 |
Target: 5'- aGGCCGC-AGACGGUgUcCGU-ACCGu -3' miRNA: 3'- -CCGGUGuUCUGCCGgAuGCAcUGGCu -5' |
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28466 | 3' | -55.8 | NC_005946.1 | + | 78930 | 0.66 | 0.845111 |
Target: 5'- aGCCu---GugGGCCUugGUGGCg-- -3' miRNA: 3'- cCGGuguuCugCCGGAugCACUGgcu -5' |
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28466 | 3' | -55.8 | NC_005946.1 | + | 45088 | 0.66 | 0.853181 |
Target: 5'- gGGCCACAgAGGUGGCCaaaaugUugGUGAUUGc -3' miRNA: 3'- -CCGGUGU-UCUGCCGG------AugCACUGGCu -5' |
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28466 | 3' | -55.8 | NC_005946.1 | + | 52554 | 0.66 | 0.853181 |
Target: 5'- gGGCUagggACAAGGCcauGGCagcgGCGuUGGCCGAg -3' miRNA: 3'- -CCGG----UGUUCUG---CCGga--UGC-ACUGGCU- -5' |
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28466 | 3' | -55.8 | NC_005946.1 | + | 92282 | 0.66 | 0.853181 |
Target: 5'- aGGUCAgGAgGAUGGCCgggcagaACcUGGCCGAg -3' miRNA: 3'- -CCGGUgUU-CUGCCGGa------UGcACUGGCU- -5' |
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28466 | 3' | -55.8 | NC_005946.1 | + | 32647 | 0.66 | 0.876123 |
Target: 5'- uGGCCaACAAGAgGGUggACGccACCGGg -3' miRNA: 3'- -CCGG-UGUUCUgCCGgaUGCacUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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