Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28557 | 3' | -61.6 | NC_005946.1 | + | 24410 | 0.66 | 0.604061 |
Target: 5'- uUGGCCacgguCUCuGCGGcCuUCUCCCacgcgcUCCCCg -3' miRNA: 3'- -ACCGG-----GAG-CGUCaGcAGAGGG------AGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 48647 | 0.66 | 0.598051 |
Target: 5'- aGGCCCccaUCGCAGaaggGUCcaaggccauggccgCCCUCUCCu -3' miRNA: 3'- aCCGGG---AGCGUCag--CAGa-------------GGGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 22494 | 0.66 | 0.594049 |
Target: 5'- gGGCCCU-GUaAGUCGg--UCCUCCUCu -3' miRNA: 3'- aCCGGGAgCG-UCAGCagaGGGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 44540 | 0.66 | 0.594049 |
Target: 5'- cGGCCCUgGCGGUgugaggCGUCUUgaggggagCCUCgUCg -3' miRNA: 3'- aCCGGGAgCGUCA------GCAGAG--------GGAGgGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 26614 | 0.66 | 0.584063 |
Target: 5'- gGGUCCagGUAGUCcUUUgCCgUCCCCg -3' miRNA: 3'- aCCGGGagCGUCAGcAGA-GGgAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 62728 | 0.66 | 0.574111 |
Target: 5'- gUGGCCCUCuugacgaCGG-CGUCgUCCUUCUUCa -3' miRNA: 3'- -ACCGGGAGc------GUCaGCAG-AGGGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 35928 | 0.66 | 0.574111 |
Target: 5'- gGGCCCUCGCAGcgggaCGUUUggaaguccuggCCgUCCUg -3' miRNA: 3'- aCCGGGAGCGUCa----GCAGA-----------GGgAGGGg -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 11067 | 0.66 | 0.573118 |
Target: 5'- aGGgUCUCGUAcacgucccacacGUCGUCUguccucaCCCUCuCCCa -3' miRNA: 3'- aCCgGGAGCGU------------CAGCAGA-------GGGAG-GGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 3745 | 0.67 | 0.525064 |
Target: 5'- gGGCCaCgaccaugaCGCcGUCGUCguccgaCCCUCCCa -3' miRNA: 3'- aCCGG-Ga-------GCGuCAGCAGa-----GGGAGGGg -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 89721 | 0.67 | 0.525064 |
Target: 5'- cGGCCCUuuccaccuuaaaUGCcG-CGUCUCCCUUuacggCCCu -3' miRNA: 3'- aCCGGGA------------GCGuCaGCAGAGGGAG-----GGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 73566 | 0.67 | 0.515436 |
Target: 5'- cGGCCCUCcucauggaGCAGcacgCGUCcgCCCccgaggaCCCCg -3' miRNA: 3'- aCCGGGAG--------CGUCa---GCAGa-GGGa------GGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 22316 | 0.67 | 0.496401 |
Target: 5'- -cGCuCCUcCGUAGUCGgcacaCUCCCUCUCg -3' miRNA: 3'- acCG-GGA-GCGUCAGCa----GAGGGAGGGg -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 71771 | 0.67 | 0.496401 |
Target: 5'- cGGUCCUgGUGGggaaggUGUCUCaCUCCCUg -3' miRNA: 3'- aCCGGGAgCGUCa-----GCAGAGgGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 80349 | 0.68 | 0.477689 |
Target: 5'- gGGCCCUCcgacguggaaGcCAGUCacgggcaUCCCUCCCUc -3' miRNA: 3'- aCCGGGAG----------C-GUCAGcag----AGGGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 16610 | 0.68 | 0.468465 |
Target: 5'- uUGGCCCcgucaaCGCAGa---CUCuCCUCCCCu -3' miRNA: 3'- -ACCGGGa-----GCGUCagcaGAG-GGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 95152 | 0.68 | 0.450296 |
Target: 5'- aGGUCCUCGCGGgCaUCUCCag-CCCg -3' miRNA: 3'- aCCGGGAGCGUCaGcAGAGGgagGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 35764 | 0.68 | 0.44047 |
Target: 5'- cUGGCCCUCGUauAGUCcccguagGUCaccacagCCCacuuaUCCCCg -3' miRNA: 3'- -ACCGGGAGCG--UCAG-------CAGa------GGG-----AGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 94551 | 0.68 | 0.438696 |
Target: 5'- aGGCaCCUccagagcuuuggagCGaCGGcggugucaUCGUCUCCUUCCCCu -3' miRNA: 3'- aCCG-GGA--------------GC-GUC--------AGCAGAGGGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 75736 | 0.68 | 0.436044 |
Target: 5'- gGGCCCUCGCAauaucggcccugauGUCGUuggaCUCcaggaugucggcggCCUCUCCc -3' miRNA: 3'- aCCGGGAGCGU--------------CAGCA----GAG--------------GGAGGGG- -5' |
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28557 | 3' | -61.6 | NC_005946.1 | + | 102605 | 0.68 | 0.431643 |
Target: 5'- aUGG-CCUCGUAGUC-UUUCCUccuggacuccaugUCCCCg -3' miRNA: 3'- -ACCgGGAGCGUCAGcAGAGGG-------------AGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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