Results 1 - 20 of 120 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28780 | 3' | -62.5 | NC_006146.1 | + | 137697 | 0.66 | 0.63328 |
Target: 5'- -cGGCucccCCUCCGGccUCCCCccgGGGUGCCc- -3' miRNA: 3'- guCCG----GGAGGUC--AGGGG---CCUACGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 55531 | 0.66 | 0.63328 |
Target: 5'- cCAGGgCCUCCAGcUCCgCCGccacgccgGCCAGg -3' miRNA: 3'- -GUCCgGGAGGUC-AGG-GGCcua-----CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 50065 | 0.66 | 0.624385 |
Target: 5'- gCAGGCCCUCCAGgaagcgcuugugcggCaCCUGGAucugacuccgguUGCgGAg -3' miRNA: 3'- -GUCCGGGAGGUCa--------------G-GGGCCU------------ACGgUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 46045 | 0.66 | 0.623396 |
Target: 5'- gGGGCCCUCCAGgggacgCagCGaGUGCCAu -3' miRNA: 3'- gUCCGGGAGGUCa-----GggGCcUACGGUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 107897 | 0.66 | 0.623396 |
Target: 5'- uCGGGCaCCcgCCAGaCuCCCGGAgcccaggGCCAGg -3' miRNA: 3'- -GUCCG-GGa-GGUCaG-GGGCCUa------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 48046 | 0.66 | 0.603654 |
Target: 5'- uCGGGUCgUCCAGcgCCCCGGGcagcgUGCgGu -3' miRNA: 3'- -GUCCGGgAGGUCa-GGGGCCU-----ACGgUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 1154 | 0.66 | 0.593809 |
Target: 5'- aGGGUCC-CCGGgccgCCCCGGGgcucccccgcGCCGAu -3' miRNA: 3'- gUCCGGGaGGUCa---GGGGCCUa---------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 3018 | 0.66 | 0.593809 |
Target: 5'- aGGGUCC-CCGGgccgCCCCGGGgcucccccgcGCCGAu -3' miRNA: 3'- gUCCGGGaGGUCa---GGGGCCUa---------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 2086 | 0.66 | 0.593809 |
Target: 5'- aGGGUCC-CCGGgccgCCCCGGGgcucccccgcGCCGAu -3' miRNA: 3'- gUCCGGGaGGUCa---GGGGCCUa---------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 57455 | 0.66 | 0.592825 |
Target: 5'- cCGGGCCgcggcucacguuuUUCCAGUCguCCCGcgggcGAUGCCAGa -3' miRNA: 3'- -GUCCGG-------------GAGGUCAG--GGGC-----CUACGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 84978 | 0.66 | 0.583989 |
Target: 5'- gCAGGCCaUCCAGgCCCCaGGGUaaaGCCc- -3' miRNA: 3'- -GUCCGGgAGGUCaGGGG-CCUA---CGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 130025 | 0.66 | 0.583989 |
Target: 5'- gAGGCCCcggcUCC-GUCUCCGGAccgcgaGCCGGg -3' miRNA: 3'- gUCCGGG----AGGuCAGGGGCCUa-----CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 52619 | 0.67 | 0.574203 |
Target: 5'- uGGGCCauCUCguGcCCCCugaGGAUGCCGc -3' miRNA: 3'- gUCCGG--GAGguCaGGGG---CCUACGGUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 24114 | 0.67 | 0.574203 |
Target: 5'- cCAGGCCCUCCgAGgCCCCag--GCCu- -3' miRNA: 3'- -GUCCGGGAGG-UCaGGGGccuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 14881 | 0.67 | 0.574203 |
Target: 5'- cCAGGCCCUCCgAGgCCCCag--GCCu- -3' miRNA: 3'- -GUCCGGGAGG-UCaGGGGccuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 27192 | 0.67 | 0.574203 |
Target: 5'- cCAGGCCCUCCgAGgCCCCag--GCCu- -3' miRNA: 3'- -GUCCGGGAGG-UCaGGGGccuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 153947 | 0.67 | 0.574203 |
Target: 5'- aAGGCCCgCCGcUCCCCGuugggcacGGUGCCu- -3' miRNA: 3'- gUCCGGGaGGUcAGGGGC--------CUACGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 131945 | 0.67 | 0.574203 |
Target: 5'- gGGGCCCa------CCCGGAUGCCAAg -3' miRNA: 3'- gUCCGGGaggucagGGGCCUACGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 52436 | 0.67 | 0.574203 |
Target: 5'- --uGCCCUCCAGUuuggggucgacgCCCUGGAgagggGCCu- -3' miRNA: 3'- gucCGGGAGGUCA------------GGGGCCUa----CGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 21036 | 0.67 | 0.574203 |
Target: 5'- cCAGGCCCUCCgAGgCCCCag--GCCu- -3' miRNA: 3'- -GUCCGGGAGG-UCaGGGGccuaCGGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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