Results 1 - 20 of 93 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28792 | 5' | -56.5 | NC_006146.1 | + | 127490 | 0.66 | 0.926291 |
Target: 5'- aCGAGGAGa-CGGCGGGgGuCGcagccGGGGCa -3' miRNA: 3'- -GCUCCUCgaGUUGCUCgC-GCa----CCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 48942 | 0.66 | 0.926291 |
Target: 5'- uCGGGGAccaGcCUCAGgGAGuUGUG-GGAGCg -3' miRNA: 3'- -GCUCCU---C-GAGUUgCUC-GCGCaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 123358 | 0.66 | 0.926291 |
Target: 5'- cCGAgGGAGCUCuGGCGcuGGC-CG-GGGGCc -3' miRNA: 3'- -GCU-CCUCGAG-UUGC--UCGcGCaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 122174 | 0.66 | 0.925764 |
Target: 5'- aGaAGGAGCUgCugcGCGAGCgggaggccagagaGCGgacGGAGCg -3' miRNA: 3'- gC-UCCUCGA-Gu--UGCUCG-------------CGCa--CCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 128199 | 0.66 | 0.925764 |
Target: 5'- aGGGGGGCgccgCGgcgccgggccagACGcucuuucGGCGCGgGGAGCu -3' miRNA: 3'- gCUCCUCGa---GU------------UGC-------UCGCGCaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 154601 | 0.66 | 0.924702 |
Target: 5'- aGAGGGGCagagccagcCAGCGgcugaacccgaggaGGCGCccGGAGCg -3' miRNA: 3'- gCUCCUCGa--------GUUGC--------------UCGCGcaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 151523 | 0.66 | 0.924702 |
Target: 5'- aGAGGGGCagagccagcCAGCGgcugaacccgaggaGGCGCccGGAGCg -3' miRNA: 3'- gCUCCUCGa--------GUUGC--------------UCGCGcaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 148445 | 0.66 | 0.924702 |
Target: 5'- aGAGGGGCagagccagcCAGCGgcugaacccgaggaGGCGCccGGAGCg -3' miRNA: 3'- gCUCCUCGa--------GUUGC--------------UCGCGcaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 145367 | 0.66 | 0.924702 |
Target: 5'- aGAGGGGCagagccagcCAGCGgcugaacccgaggaGGCGCccGGAGCg -3' miRNA: 3'- gCUCCUCGa--------GUUGC--------------UCGCGcaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 142289 | 0.66 | 0.924702 |
Target: 5'- aGAGGGGCagagccagcCAGCGgcugaacccgaggaGGCGCccGGAGCg -3' miRNA: 3'- gCUCCUCGa--------GUUGC--------------UCGCGcaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 57972 | 0.66 | 0.923092 |
Target: 5'- aCGAGGAGUUCuuccaggcgcaccagGACGAGC---UGGAGg -3' miRNA: 3'- -GCUCCUCGAG---------------UUGCUCGcgcACCUCg -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 43626 | 0.66 | 0.920913 |
Target: 5'- aGAgGGAGUgggCGGCGGG-GCGUGGccGCc -3' miRNA: 3'- gCU-CCUCGa--GUUGCUCgCGCACCu-CG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 53331 | 0.66 | 0.920913 |
Target: 5'- cCGAGGAgGCcCGGCG-GC-CGcGGGGCg -3' miRNA: 3'- -GCUCCU-CGaGUUGCuCGcGCaCCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 130722 | 0.66 | 0.920913 |
Target: 5'- cCGAGGAGgaCGAgGAG-GCG-GcGGGCu -3' miRNA: 3'- -GCUCCUCgaGUUgCUCgCGCaC-CUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 51185 | 0.66 | 0.920913 |
Target: 5'- -aAGGGGgUCuGCGGGCugGCGUGGuacagGGCa -3' miRNA: 3'- gcUCCUCgAGuUGCUCG--CGCACC-----UCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 101064 | 0.66 | 0.915303 |
Target: 5'- cCGAGG-GC-C-ACGGGCucauGCuGUGGGGCa -3' miRNA: 3'- -GCUCCuCGaGuUGCUCG----CG-CACCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 161430 | 0.66 | 0.915303 |
Target: 5'- gCGGGuGuGGCcCcGCGggaacccaaGGCGCGUGGGGCg -3' miRNA: 3'- -GCUC-C-UCGaGuUGC---------UCGCGCACCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 169650 | 0.66 | 0.915303 |
Target: 5'- gCGGGGAGaauaCAGCuGGGCGUGgcgGGcGCg -3' miRNA: 3'- -GCUCCUCga--GUUG-CUCGCGCa--CCuCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 61586 | 0.66 | 0.914729 |
Target: 5'- uGAGGAagagccgGCUgCAGCGGcCGCGgccGGGGCc -3' miRNA: 3'- gCUCCU-------CGA-GUUGCUcGCGCa--CCUCG- -5' |
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28792 | 5' | -56.5 | NC_006146.1 | + | 13945 | 0.66 | 0.913575 |
Target: 5'- gGGGGAGCccaggacaacuuuaUCAGCGAGC----GGAGCc -3' miRNA: 3'- gCUCCUCG--------------AGUUGCUCGcgcaCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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