Results 1 - 20 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2884 | 3' | -54.3 | NC_001493.1 | + | 126342 | 0.66 | 0.946235 |
Target: 5'- -cGUC-CCGaGaGUCUCGACCgcGGAggaCCCg -3' miRNA: 3'- uuUAGaGGC-C-CAGAGCUGG--UCUa--GGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 94786 | 0.66 | 0.946235 |
Target: 5'- gGGAUCUCCcGGaacagCUCGACCGca-CCCa -3' miRNA: 3'- -UUUAGAGGcCCa----GAGCUGGUcuaGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 10788 | 0.66 | 0.946235 |
Target: 5'- -cGUC-CCGaGaGUCUCGACCgcGGAggaCCCg -3' miRNA: 3'- uuUAGaGGC-C-CAGAGCUGG--UCUa--GGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 25458 | 0.66 | 0.941662 |
Target: 5'- cGGUCUCCGGGgggaUCaACCGcgugcGAUCCUc -3' miRNA: 3'- uUUAGAGGCCCag--AGcUGGU-----CUAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 48143 | 0.66 | 0.936845 |
Target: 5'- -uGUCUCuCGGGUUgaagUCGACacccAUCCCg -3' miRNA: 3'- uuUAGAG-GCCCAG----AGCUGguc-UAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 131655 | 0.66 | 0.936845 |
Target: 5'- -cGUC-CCGGGgCUCGACCgcucgAGAcUCCUc -3' miRNA: 3'- uuUAGaGGCCCaGAGCUGG-----UCU-AGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 16100 | 0.66 | 0.936845 |
Target: 5'- -cGUC-CCGGGgCUCGACCgcucgAGAcUCCUc -3' miRNA: 3'- uuUAGaGGCCCaGAGCUGG-----UCU-AGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 110501 | 0.66 | 0.931781 |
Target: 5'- --uUCUUCGGGaUCUCGACCcaccGAUgCg -3' miRNA: 3'- uuuAGAGGCCC-AGAGCUGGu---CUAgGg -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 126869 | 0.66 | 0.931262 |
Target: 5'- cAGAUCgUCCgccucggGGGUCaUCGAgcCCuGGUCCCg -3' miRNA: 3'- -UUUAG-AGG-------CCCAG-AGCU--GGuCUAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 11315 | 0.66 | 0.931262 |
Target: 5'- cAGAUCgUCCgccucggGGGUCaUCGAgcCCuGGUCCCg -3' miRNA: 3'- -UUUAG-AGG-------CCCAG-AGCU--GGuCUAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 23375 | 0.66 | 0.92647 |
Target: 5'- -uGUCUUCGGuGgcCUCGGCCAGcAUCUg -3' miRNA: 3'- uuUAGAGGCC-Ca-GAGCUGGUC-UAGGg -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 37553 | 0.66 | 0.92647 |
Target: 5'- -------gGGGUCgUCGACCGGGUCgCCc -3' miRNA: 3'- uuuagaggCCCAG-AGCUGGUCUAG-GG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 95489 | 0.66 | 0.92647 |
Target: 5'- cGAUCggggaggugaCGGGUCguuucugguUCGACgAGGUCCCc -3' miRNA: 3'- uUUAGag--------GCCCAG---------AGCUGgUCUAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 32513 | 0.67 | 0.920912 |
Target: 5'- uAGGUUga-GGGUCUCGACC---UCCCu -3' miRNA: 3'- -UUUAGaggCCCAGAGCUGGucuAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 110354 | 0.67 | 0.915105 |
Target: 5'- ---aCUCCGGGcgcgggCUCGACCuucAGGUCa- -3' miRNA: 3'- uuuaGAGGCCCa-----GAGCUGG---UCUAGgg -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 46347 | 0.67 | 0.909053 |
Target: 5'- --uUCUCCGGGUgg-GACCGucuUCCCg -3' miRNA: 3'- uuuAGAGGCCCAgagCUGGUcu-AGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 90839 | 0.67 | 0.895551 |
Target: 5'- --cUCUCgacucggagccggCGGGcCUCGAUCAGAUCUUu -3' miRNA: 3'- uuuAGAG-------------GCCCaGAGCUGGUCUAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 126258 | 0.67 | 0.889441 |
Target: 5'- aAAAUCUCUaaGUCUC-ACCcGAUCCCg -3' miRNA: 3'- -UUUAGAGGccCAGAGcUGGuCUAGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 72937 | 0.68 | 0.885262 |
Target: 5'- uGAGUCUUuaCGGGuacccccaUCUCGACCAGAgcgggaagaagugcgUCUCg -3' miRNA: 3'- -UUUAGAG--GCCC--------AGAGCUGGUCU---------------AGGG- -5' |
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2884 | 3' | -54.3 | NC_001493.1 | + | 126154 | 0.68 | 0.88243 |
Target: 5'- aAAAUCUCUaaGUCUCgGACCGGGgcugugCCCa -3' miRNA: 3'- -UUUAGAGGccCAGAG-CUGGUCUa-----GGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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