Results 1 - 20 of 97 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2887 | 3' | -60.5 | NC_001493.1 | + | 19263 | 0.66 | 0.720682 |
Target: 5'- gCGGGuGCGCCAUCUCaaaGUCGaugaGCCc -3' miRNA: 3'- aGCUCuCGCGGUGGGGg--UAGCgg--UGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 27822 | 0.66 | 0.720682 |
Target: 5'- uUCGAGucCGgCACCggaucgacacuCUCAUCGUCACCc -3' miRNA: 3'- -AGCUCucGCgGUGG-----------GGGUAGCGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 53205 | 0.66 | 0.714885 |
Target: 5'- cUCGAGAcgagcgagcacaaccGCGUaagaUACCCCCGUacuccCGCCuCCg -3' miRNA: 3'- -AGCUCU---------------CGCG----GUGGGGGUA-----GCGGuGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 129948 | 0.66 | 0.711007 |
Target: 5'- cCGuGAGguuaGCgGCCCCCG-CGUCGCUc -3' miRNA: 3'- aGCuCUCg---CGgUGGGGGUaGCGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 90209 | 0.66 | 0.711007 |
Target: 5'- cCGGaauGCGCUAUaCCCCAaaugCGCCAUCg -3' miRNA: 3'- aGCUcu-CGCGGUG-GGGGUa---GCGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 14394 | 0.66 | 0.711007 |
Target: 5'- cCGuGAGguuaGCgGCCCCCG-CGUCGCUc -3' miRNA: 3'- aGCuCUCg---CGgUGGGGGUaGCGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 126201 | 0.66 | 0.711007 |
Target: 5'- cUCGGGAcGCGCCcgaucgcgcgaGCuCCCCGgguUCGaCACCc -3' miRNA: 3'- -AGCUCU-CGCGG-----------UG-GGGGU---AGCgGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 10647 | 0.66 | 0.711007 |
Target: 5'- cUCGGGAcGCGCCcgaucgcgcgaGCuCCCCGgguUCGaCACCc -3' miRNA: 3'- -AGCUCU-CGCGG-----------UG-GGGGU---AGCgGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 62066 | 0.66 | 0.70127 |
Target: 5'- aUCGAGAGUuuaucaggggcuGUCACUaCCgGUUGCCgACCg -3' miRNA: 3'- -AGCUCUCG------------CGGUGG-GGgUAGCGG-UGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 123605 | 0.66 | 0.70127 |
Target: 5'- cCG-GGGCGUCcguugaaaucgACCCUCGUUucgggGCCACCg -3' miRNA: 3'- aGCuCUCGCGG-----------UGGGGGUAG-----CGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 8051 | 0.66 | 0.70127 |
Target: 5'- cCG-GGGCGUCcguugaaaucgACCCUCGUUucgggGCCACCg -3' miRNA: 3'- aGCuCUCGCGG-----------UGGGGGUAG-----CGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 60442 | 0.66 | 0.70127 |
Target: 5'- cCGcGGGCGCgAUCCCCAgucugaCGCgUACCc -3' miRNA: 3'- aGCuCUCGCGgUGGGGGUa-----GCG-GUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 27971 | 0.66 | 0.70127 |
Target: 5'- aUCGGGGcacGCGCgGCacccgaaCCCGUaucccaguuacCGCCGCCa -3' miRNA: 3'- -AGCUCU---CGCGgUGg------GGGUA-----------GCGGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 38380 | 0.66 | 0.691476 |
Target: 5'- ----cAGCGUgGCUCCCAUgGaCCACCg -3' miRNA: 3'- agcucUCGCGgUGGGGGUAgC-GGUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 124056 | 0.66 | 0.685578 |
Target: 5'- gCGAGAcGCGCgGCCCCUccacgguucgaagcgGgcacggcgCGCCACg -3' miRNA: 3'- aGCUCU-CGCGgUGGGGG---------------Ua-------GCGGUGg -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 128226 | 0.66 | 0.68065 |
Target: 5'- gCGAguaucccGAGCGCCccgACCCCgGaCGCgACCc -3' miRNA: 3'- aGCU-------CUCGCGG---UGGGGgUaGCGgUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 12672 | 0.66 | 0.68065 |
Target: 5'- gCGAguaucccGAGCGCCccgACCCCgGaCGCgACCc -3' miRNA: 3'- aGCU-------CUCGCGG---UGGGGgUaGCGgUGG- -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 8501 | 0.66 | 0.678677 |
Target: 5'- gCGAGAcGCGCgGCCCCUccacguucgaagcgGgcacggcgCGCCACg -3' miRNA: 3'- aGCUCU-CGCGgUGGGGG--------------Ua-------GCGGUGg -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 61562 | 0.66 | 0.678677 |
Target: 5'- aCGGGGcGCGCCAUCUCUggcuccucguccgaGUCGCuCACg -3' miRNA: 3'- aGCUCU-CGCGGUGGGGG--------------UAGCG-GUGg -5' |
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2887 | 3' | -60.5 | NC_001493.1 | + | 130501 | 0.66 | 0.671759 |
Target: 5'- -gGAGGGUGCCAUagcgCUCC--CGCUACCg -3' miRNA: 3'- agCUCUCGCGGUG----GGGGuaGCGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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