Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29173 | 5' | -54.1 | NC_006150.1 | + | 165122 | 0.66 | 0.985301 |
Target: 5'- cGAUCAcCGUGGuGCUCAUUuuccuuauAGGUGCa -3' miRNA: 3'- cCUGGUaGCGCU-CGAGUAGu-------UCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 135538 | 0.66 | 0.985301 |
Target: 5'- uGGuGCCGcCGCGguGGUggaCGUCGAGGCGg -3' miRNA: 3'- -CC-UGGUaGCGC--UCGa--GUAGUUCCGCg -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 129614 | 0.66 | 0.983529 |
Target: 5'- aGGCUGUgCGUGGacGCUUcgUggGGCGCg -3' miRNA: 3'- cCUGGUA-GCGCU--CGAGuaGuuCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 106706 | 0.66 | 0.983529 |
Target: 5'- gGGGCCAgucggcaCGCGAccGaCUCggCGGGaGCGCg -3' miRNA: 3'- -CCUGGUa------GCGCU--C-GAGuaGUUC-CGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 46369 | 0.66 | 0.981599 |
Target: 5'- cGACCAacaagUgGCGGuGCUCAUCGgaaaacaaguccGGGCGa -3' miRNA: 3'- cCUGGU-----AgCGCU-CGAGUAGU------------UCCGCg -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 106882 | 0.66 | 0.981599 |
Target: 5'- uGGGCCAgUGCcaagGAcGCUUGagAGGGCGCg -3' miRNA: 3'- -CCUGGUaGCG----CU-CGAGUagUUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 119526 | 0.67 | 0.974793 |
Target: 5'- uGGACgCGgagCGCGuucGCggCGUCuuuGGCGCg -3' miRNA: 3'- -CCUG-GUa--GCGCu--CGa-GUAGuu-CCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 48890 | 0.67 | 0.970491 |
Target: 5'- uGGugCGUUGCGuGGCUCGuauaguguguguuaaUCAauugcucgGGGUGCu -3' miRNA: 3'- -CCugGUAGCGC-UCGAGU---------------AGU--------UCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 219768 | 0.67 | 0.969338 |
Target: 5'- aGGACCuggcguggcCGCGGGUggaaCGUCcgaggcGGGCGCg -3' miRNA: 3'- -CCUGGua-------GCGCUCGa---GUAGu-----UCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 144914 | 0.67 | 0.966315 |
Target: 5'- --uUCAUCGCGgaccGGC-CG-CGAGGCGCa -3' miRNA: 3'- ccuGGUAGCGC----UCGaGUaGUUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 139365 | 0.67 | 0.966315 |
Target: 5'- -cACCGaaGCG-GUUCG-CAAGGCGCa -3' miRNA: 3'- ccUGGUagCGCuCGAGUaGUUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 217279 | 0.68 | 0.952134 |
Target: 5'- cGGAUgGggagCGCGuugacauuccGCUUGUCGAGGCGUu -3' miRNA: 3'- -CCUGgUa---GCGCu---------CGAGUAGUUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 88754 | 0.68 | 0.952134 |
Target: 5'- cGAuCCAgcacugCGUccaGGGCUCGUCGGGGCaGCc -3' miRNA: 3'- cCU-GGUa-----GCG---CUCGAGUAGUUCCG-CG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 105997 | 0.68 | 0.948043 |
Target: 5'- cGGCCuccUCGgGAGcCUCGUCGuaGCGCa -3' miRNA: 3'- cCUGGu--AGCgCUC-GAGUAGUucCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 8885 | 0.68 | 0.948043 |
Target: 5'- --cCCAaaaG-GAGCUCcUCAAGGCGCg -3' miRNA: 3'- ccuGGUag-CgCUCGAGuAGUUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 75221 | 0.69 | 0.934414 |
Target: 5'- uGACUuuaGaCGAGCUCAagaugCAGGGCGUg -3' miRNA: 3'- cCUGGuagC-GCUCGAGUa----GUUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 88583 | 0.69 | 0.929411 |
Target: 5'- gGGGgCGUCGCGAgGCcCAgcuGGGGUGCa -3' miRNA: 3'- -CCUgGUAGCGCU-CGaGUag-UUCCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 85900 | 0.69 | 0.924178 |
Target: 5'- uGAuCCAcCGUGAGCccCAUCAAgagcGGCGCa -3' miRNA: 3'- cCU-GGUaGCGCUCGa-GUAGUU----CCGCG- -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 55626 | 0.7 | 0.907095 |
Target: 5'- uGGACCAcaguguggUCaGCGAGCUCAUUAAG-UGg -3' miRNA: 3'- -CCUGGU--------AG-CGCUCGAGUAGUUCcGCg -5' |
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29173 | 5' | -54.1 | NC_006150.1 | + | 183514 | 0.7 | 0.881157 |
Target: 5'- cGGAUUcugCGUGGGCUCcggugCGGGGUGCg -3' miRNA: 3'- -CCUGGua-GCGCUCGAGua---GUUCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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