Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 29377 | 5' | -57.2 | NC_006151.1 | + | 55610 | 0.66 | 0.842657 |
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Target: 5'- cGGACguggaGCGcCUCUCGCGGCUcuGGGaGCAg -3' miRNA: 3'- -CCUGa----UGUaGAGGGUGCUGG--CCC-CGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 57521 | 0.66 | 0.850561 |
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Target: 5'- cGGCgGCGgccgcCUCCggCGCGGCCGcGGGCAc -3' miRNA: 3'- cCUGaUGUa----GAGG--GUGCUGGC-CCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 5902 | 0.66 | 0.857509 |
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Target: 5'- -cGCUGCGagugCUgCCggggucgGCGGCCGGGGCc -3' miRNA: 3'- ccUGAUGUa---GAgGG-------UGCUGGCCCCGu -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 73199 | 0.66 | 0.857509 |
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Target: 5'- cGACUGCug--CCCGCGGgccaggcCCGcGGGCAc -3' miRNA: 3'- cCUGAUGuagaGGGUGCU-------GGC-CCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 132560 | 0.66 | 0.880182 |
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Target: 5'- cGGGCaGCuccUCcUCCACGGCCGGGacucGCGg -3' miRNA: 3'- -CCUGaUGu--AGaGGGUGCUGGCCC----CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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