miRNA: 3'- cCuGAUGUAGag---GGUGCuGGCCCCGU- -5'
miRNA: 3'- -CCUGa--UGUa---GAGGG-----UGCUGGCCCCGU- -5'
miRNA: 3'- -CCUGaUG--UAGaGGGUGCUGgCCCCGU- -5'
miRNA: 3'- -CCUGAUGuAGaGGGUgCUGGCCCCGu -5'
miRNA: 3'- -CCUGAUGUAGAGGGUGCUGGCCCCGU- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29377 | 5' | -57.2 | NC_006151.1 | + | 62451 | 0.74 | 0.406834 |
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Target: 5'- cGcCUGCAUCgcccaCCGCGcCCGGGGCAg -3' miRNA: 3'- cCuGAUGUAGag---GGUGCuGGCCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 637 | 0.75 | 0.398375 |
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Target: 5'- cGGACgugACGccggCUUCCggggcGCGGCCGGGGCGg -3' miRNA: 3'- -CCUGa--UGUa---GAGGG-----UGCUGGCCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 90676 | 0.76 | 0.342419 |
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Target: 5'- cGGGCcGCuuGUCcCCCGCGGCgGGGGCGg -3' miRNA: 3'- -CCUGaUG--UAGaGGGUGCUGgCCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 27333 | 0.78 | 0.248204 |
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Target: 5'- gGGGCUGC-UC-CCCGgGACCGGGGUc -3' miRNA: 3'- -CCUGAUGuAGaGGGUgCUGGCCCCGu -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 136500 | 1.1 | 0.001864 |
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Target: 5'- gGGACUACAUCUCCCACGACCGGGGCAg -3' miRNA: 3'- -CCUGAUGUAGAGGGUGCUGGCCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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