Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29380 | 5' | -49.7 | NC_006151.1 | + | 24053 | 0.68 | 0.978591 |
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Target: 5'- uGGAGAUGCGCa-----AAGGUGUGUg -3' miRNA: 3'- uCUUCUACGCGaacaacUUCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 23010 | 0.69 | 0.967198 |
|
Target: 5'- gGGuGGggGCGCgggggUGgUGGAGGCG-GCg -3' miRNA: 3'- -UCuUCuaCGCGa----ACaACUUCCGCaCG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 20876 | 0.66 | 0.996428 |
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Target: 5'- uAGcAGgcGCGCUUGggGucGAGGCGcaGCa -3' miRNA: 3'- -UCuUCuaCGCGAACaaC--UUCCGCa-CG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 15032 | 0.66 | 0.995516 |
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Target: 5'- gAGGAGGUGCGCguccac--GGCGcGCg -3' miRNA: 3'- -UCUUCUACGCGaacaacuuCCGCaCG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 11730 | 0.67 | 0.993323 |
|
Target: 5'- gGGggGAgcggGgGUgaGggGGAGGgGUGCg -3' miRNA: 3'- -UCuuCUa---CgCGaaCaaCUUCCgCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 8612 | 0.67 | 0.99111 |
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Target: 5'- gGGccGGUGCGggUG-UGAGGGUccuGUGCg -3' miRNA: 3'- -UCuuCUACGCgaACaACUUCCG---CACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 8564 | 0.66 | 0.996428 |
|
Target: 5'- gAGAGcGUGCGUgugggugggUGggugUGAGGGCcgGUGCg -3' miRNA: 3'- -UCUUcUACGCGa--------ACa---ACUUCCG--CACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 8533 | 0.71 | 0.915633 |
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Target: 5'- gGGGAGA-GUGUgaUGUgGggGGUGUGCg -3' miRNA: 3'- -UCUUCUaCGCGa-ACAaCuuCCGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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