Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29448 | 5' | -50.1 | NC_006151.1 | + | 26286 | 0.7 | 0.934709 |
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Target: 5'- gGgcGGACggUGggGUGGGGGggGUGGg -3' miRNA: 3'- aCuuCUUGa-ACuuCGUCCCCgaCAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 33324 | 0.7 | 0.934709 |
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Target: 5'- gGAccGGGACagGggGCGGGGGCg--GGg -3' miRNA: 3'- aCU--UCUUGaaCuuCGUCCCCGacaUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 32978 | 0.71 | 0.905071 |
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Target: 5'- gGGAGAGCgcgagagUGAGGCuguuGGCUGUGGg -3' miRNA: 3'- aCUUCUUGa------ACUUCGucc-CCGACAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 66916 | 0.72 | 0.860906 |
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Target: 5'- aGGAGGGCcacgaugGAGGCGGGGGCg--AGg -3' miRNA: 3'- aCUUCUUGaa-----CUUCGUCCCCGacaUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 29665 | 0.72 | 0.852696 |
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Target: 5'- aGGAGGAaggGggGgGGGGGCgggGUGGg -3' miRNA: 3'- aCUUCUUgaaCuuCgUCCCCGa--CAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 80031 | 0.73 | 0.835609 |
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Target: 5'- cGAucgGGGACggggGAGGCGGGagucuGGCUGUGGg -3' miRNA: 3'- aCU---UCUUGaa--CUUCGUCC-----CCGACAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 113694 | 0.77 | 0.6324 |
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Target: 5'- cGAGGGGCgcgUGu-GCGGGGaGCUGUGGa -3' miRNA: 3'- aCUUCUUGa--ACuuCGUCCC-CGACAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 72939 | 1.11 | 0.006616 |
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Target: 5'- gUGAAGAACUUGAAGCAGGGGCUGUAGg -3' miRNA: 3'- -ACUUCUUGAACUUCGUCCCCGACAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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