Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29493 | 3' | -52.8 | NC_006151.1 | + | 72577 | 0.66 | 0.96687 |
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Target: 5'- -gGGGGCgggugaCAGAgucGGGGUgaggggGGGGGCAg -3' miRNA: 3'- caUCCCGag----GUCU---UCCCAa-----CUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 4523 | 0.66 | 0.970018 |
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Target: 5'- -cAGGGC-CCAGAGcGGGUccuGGGCGg -3' miRNA: 3'- caUCCCGaGGUCUU-CCCAacuCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 8387 | 0.66 | 0.970018 |
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Target: 5'- -gGGGGgUCCGGGAuGGGggGGGu-CAa -3' miRNA: 3'- caUCCCgAGGUCUU-CCCaaCUCuuGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 44397 | 0.66 | 0.970018 |
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Target: 5'- -gGGGGCgCCgAGGAGGGagGGGcGCGu -3' miRNA: 3'- caUCCCGaGG-UCUUCCCaaCUCuUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 45319 | 0.66 | 0.970018 |
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Target: 5'- --cGGGaggCUGGGAGGGUggcggugGAGGACGg -3' miRNA: 3'- cauCCCga-GGUCUUCCCAa------CUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 5511 | 0.66 | 0.970018 |
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Target: 5'- -cGGGGCUCCGGGcgccaAGcGGccGAGGAUc -3' miRNA: 3'- caUCCCGAGGUCU-----UC-CCaaCUCUUGu -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 121661 | 0.66 | 0.970018 |
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Target: 5'- --cGGGCgggCGGAGGGGggGAgcGAGCGg -3' miRNA: 3'- cauCCCGag-GUCUUCCCaaCU--CUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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