Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29493 | 3' | -52.8 | NC_006151.1 | + | 45461 | 0.69 | 0.86816 |
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Target: 5'- -cGGGGCggcgaCGGAcccAGGGgUGAGAGCGg -3' miRNA: 3'- caUCCCGag---GUCU---UCCCaACUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 31651 | 0.7 | 0.844019 |
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Target: 5'- --cGGGCgcgCgGGGAGGGagGAGGGCGg -3' miRNA: 3'- cauCCCGa--GgUCUUCCCaaCUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 8436 | 0.7 | 0.824239 |
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Target: 5'- uGUAGGGaaggcucgcucucuCUCguGGAGGGUcggGAGGACGg -3' miRNA: 3'- -CAUCCC--------------GAGguCUUCCCAa--CUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 5257 | 0.71 | 0.808973 |
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Target: 5'- -gGGGGC-CCGGggGGcg-GAGGGCGa -3' miRNA: 3'- caUCCCGaGGUCuuCCcaaCUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 29277 | 0.71 | 0.799757 |
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Target: 5'- --cGGGUgagUCGGGAGGGUggggUGGGAGCGg -3' miRNA: 3'- cauCCCGa--GGUCUUCCCA----ACUCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 99485 | 0.77 | 0.481602 |
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Target: 5'- cUGGGGCUCCccgAGAAGGGcgUG-GAGCAc -3' miRNA: 3'- cAUCCCGAGG---UCUUCCCa-ACuCUUGU- -5' |
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| 29493 | 3' | -52.8 | NC_006151.1 | + | 43132 | 1.08 | 0.005273 |
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Target: 5'- gGUAGGGCUCCAGAAGGGUUGAGAACAc -3' miRNA: 3'- -CAUCCCGAGGUCUUCCCAACUCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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