Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30418 | 5' | -54.8 | NC_006548.1 | + | 4303 | 0.67 | 0.551109 |
Target: 5'- aGCACGGuGCAgAGCggcagaugGGUAGCUa- -3' miRNA: 3'- cCGUGCCuUGUgUCGaa------CCAUCGGgg -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 21852 | 0.67 | 0.584569 |
Target: 5'- aGGCcCGGAcCuccaAGC-UGGUcuucAGCCCCa -3' miRNA: 3'- -CCGuGCCUuGug--UCGaACCA----UCGGGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 9662 | 0.67 | 0.584569 |
Target: 5'- gGGUGCGGGGCAUGGC--GGU-GUUCCu -3' miRNA: 3'- -CCGUGCCUUGUGUCGaaCCAuCGGGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 16193 | 0.67 | 0.584569 |
Target: 5'- cGGCGCGGuGCGCGGUaauaacgacuUUGGaGGCUa- -3' miRNA: 3'- -CCGUGCCuUGUGUCG----------AACCaUCGGgg -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 8215 | 0.67 | 0.594692 |
Target: 5'- cGGCagcucuacuGCGGAuuuCGGUUUGGUGGCaguacguCCCa -3' miRNA: 3'- -CCG---------UGCCUuguGUCGAACCAUCG-------GGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 20903 | 0.67 | 0.595818 |
Target: 5'- cGGCcgaGGugcuGCGCAGCUUGcUGGCCg- -3' miRNA: 3'- -CCGug-CCu---UGUGUCGAACcAUCGGgg -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 18568 | 0.75 | 0.197438 |
Target: 5'- gGGCAU-GAGCGCAGCUggcgUGGaAGCCCg -3' miRNA: 3'- -CCGUGcCUUGUGUCGA----ACCaUCGGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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