Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30420 | 5' | -58.7 | NC_006548.1 | + | 35529 | 0.67 | 0.353773 |
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Target: 5'- gGCCUGCUGGaCCAggcgucauaucGCAagGCCGcAGAu -3' miRNA: 3'- gCGGACGACC-GGU-----------UGUggUGGC-UCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 22327 | 0.66 | 0.38026 |
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Target: 5'- gGUCUGCgcggUGGCCAGCGCguCCGuGu- -3' miRNA: 3'- gCGGACG----ACCGGUUGUGguGGCuCuu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 985 | 0.66 | 0.38026 |
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Target: 5'- uCGuCCUGCaGGCCAGCugCugaCGAGc- -3' miRNA: 3'- -GC-GGACGaCCGGUUGugGug-GCUCuu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 29043 | 0.66 | 0.38026 |
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Target: 5'- aGCUUGCUgaccaGGCCAGCGCCuuCGGu-- -3' miRNA: 3'- gCGGACGA-----CCGGUUGUGGugGCUcuu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 24704 | 0.66 | 0.38026 |
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Target: 5'- aGCaCUGUcGGUgAGaACCACCGAGGAg -3' miRNA: 3'- gCG-GACGaCCGgUUgUGGUGGCUCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 16457 | 0.66 | 0.402383 |
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Target: 5'- uGCC-GCUGGCCccaGacaguuuucaccuggGCAUCGCUGAGGAu -3' miRNA: 3'- gCGGaCGACCGG---U---------------UGUGGUGGCUCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 7579 | 0.66 | 0.40804 |
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Target: 5'- uCGCCaaGUUGGCCGA-GCagGCCGAGGAg -3' miRNA: 3'- -GCGGa-CGACCGGUUgUGg-UGGCUCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 2919 | 0.66 | 0.40804 |
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Target: 5'- gCGCCUGCaggcGGCCAagGCGCUgAUCGAcGAGu -3' miRNA: 3'- -GCGGACGa---CCGGU--UGUGG-UGGCU-CUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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