Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30420 | 5' | -58.7 | NC_006548.1 | + | 28904 | 0.69 | 0.241387 |
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Target: 5'- cCGUCgagGC-GGCCGGCGCCGagaUCGAGAAg -3' miRNA: 3'- -GCGGa--CGaCCGGUUGUGGU---GGCUCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 29043 | 0.66 | 0.38026 |
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Target: 5'- aGCUUGCUgaccaGGCCAGCGCCuuCGGu-- -3' miRNA: 3'- gCGGACGA-----CCGGUUGUGGugGCUcuu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 29155 | 0.71 | 0.196748 |
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Target: 5'- gGCCUGCUGGgCGGC-CUggauacucgacuccuGCCGAGAc -3' miRNA: 3'- gCGGACGACCgGUUGuGG---------------UGGCUCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 29418 | 0.7 | 0.231336 |
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Target: 5'- aGCCgaucGCUGGCCGACGuuGCCGcgcagcagcuggcccAGAAg -3' miRNA: 3'- gCGGa---CGACCGGUUGUggUGGC---------------UCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 32402 | 0.68 | 0.304807 |
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Target: 5'- aGCCUgGCcGGCCAGCcgucGCCGCCGuuGc -3' miRNA: 3'- gCGGA-CGaCCGGUUG----UGGUGGCucUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 33033 | 0.72 | 0.164917 |
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Target: 5'- aGCCgGCcGGCCAGCGCCGCUGcaccGGGc -3' miRNA: 3'- gCGGaCGaCCGGUUGUGGUGGC----UCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 35529 | 0.67 | 0.353773 |
|
Target: 5'- gGCCUGCUGGaCCAggcgucauaucGCAagGCCGcAGAu -3' miRNA: 3'- gCGGACGACC-GGU-----------UGUggUGGC-UCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 36588 | 0.69 | 0.261213 |
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Target: 5'- aCGCC-GC-GGCCGGCACCGCggugcucgcgCGGGGAu -3' miRNA: 3'- -GCGGaCGaCCGGUUGUGGUG----------GCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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