Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30420 | 5' | -58.7 | NC_006548.1 | + | 20295 | 0.68 | 0.304807 |
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Target: 5'- uGCCUuugaUGGCCGgcgGCACggCACCGAGGAa -3' miRNA: 3'- gCGGAcg--ACCGGU---UGUG--GUGGCUCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 15209 | 0.68 | 0.282344 |
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Target: 5'- gCGCUUGCcGGCCuGCGCguCCGGGu- -3' miRNA: 3'- -GCGGACGaCCGGuUGUGguGGCUCuu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 29155 | 0.71 | 0.196748 |
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Target: 5'- gGCCUGCUGGgCGGC-CUggauacucgacuccuGCCGAGAc -3' miRNA: 3'- gCGGACGACCgGUUGuGG---------------UGGCUCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 2479 | 0.71 | 0.184226 |
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Target: 5'- gCGaCgUGCUGGCCAGCAUgGCUGGcGAAg -3' miRNA: 3'- -GC-GgACGACCGGUUGUGgUGGCU-CUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 33033 | 0.72 | 0.164917 |
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Target: 5'- aGCCgGCcGGCCAGCGCCGCUGcaccGGGc -3' miRNA: 3'- gCGGaCGaCCGGUUGUGGUGGC----UCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 12587 | 0.78 | 0.060068 |
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Target: 5'- gGCCaGCUGGUCGGCGCCggauGCCGAGGc -3' miRNA: 3'- gCGGaCGACCGGUUGUGG----UGGCUCUu -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 7755 | 1.05 | 0.000416 |
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Target: 5'- gCGCCUGCUGGCCAACACCACCGAGAAc -3' miRNA: 3'- -GCGGACGACCGGUUGUGGUGGCUCUU- -5' |
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| 30420 | 5' | -58.7 | NC_006548.1 | + | 2919 | 0.66 | 0.40804 |
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Target: 5'- gCGCCUGCaggcGGCCAagGCGCUgAUCGAcGAGu -3' miRNA: 3'- -GCGGACGa---CCGGU--UGUGG-UGGCU-CUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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