Results 1 - 20 of 61 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30424 | 3' | -58.8 | NC_006548.1 | + | 25040 | 0.65 | 0.442666 |
Target: 5'- gGCgUCGGCGGCguucaagGUGUCGUacAGCACacugCCCg -3' miRNA: 3'- gCG-AGCCGUUG-------UACGGCG--UCGUG----GGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 37517 | 0.65 | 0.442666 |
Target: 5'- aGCcgUGGCGGCAUGUacucccaaauuggCGUacuGGCACCCg -3' miRNA: 3'- gCGa-GCCGUUGUACG-------------GCG---UCGUGGGg -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 3307 | 0.66 | 0.405402 |
Target: 5'- gGCUCGGguCAugA-GCUGCAGaGCCCg -3' miRNA: 3'- gCGAGCC--GUugUaCGGCGUCgUGGGg -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 21997 | 0.66 | 0.39617 |
Target: 5'- cCGaacaUCGGCGuGCuggGCCGUcGCACCgCCa -3' miRNA: 3'- -GCg---AGCCGU-UGua-CGGCGuCGUGG-GG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 11878 | 0.66 | 0.404473 |
Target: 5'- uGCUCGGcCAACcuggaggucguagGUGCCugGCuGCGCCgCu -3' miRNA: 3'- gCGAGCC-GUUG-------------UACGG--CGuCGUGGgG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 32478 | 0.66 | 0.433894 |
Target: 5'- cCGCgaaCGGCAACG-GCgGCgacGGCugCCg -3' miRNA: 3'- -GCGa--GCCGUUGUaCGgCG---UCGugGGg -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 25717 | 0.66 | 0.414769 |
Target: 5'- uGCUggagCGGCAACuccaguaccugGUGCCGaacaGGCAaCCCa -3' miRNA: 3'- gCGA----GCCGUUG-----------UACGGCg---UCGUgGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 1477 | 0.66 | 0.424267 |
Target: 5'- uCGC-CGGCAGuggaGUgGUAGCGCUCCa -3' miRNA: 3'- -GCGaGCCGUUgua-CGgCGUCGUGGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 7206 | 0.66 | 0.433894 |
Target: 5'- uGC-CaGCGACAggcccagcucUGCCGCGGCugCUUu -3' miRNA: 3'- gCGaGcCGUUGU----------ACGGCGUCGugGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 9642 | 0.66 | 0.433894 |
Target: 5'- gCGCUCGau-GCAcUGaUCGCGuGCGCCCCu -3' miRNA: 3'- -GCGAGCcguUGU-AC-GGCGU-CGUGGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 36253 | 0.66 | 0.424267 |
Target: 5'- aGUUCGGCGGCuggaCUGCGGaCGUCCCg -3' miRNA: 3'- gCGAGCCGUUGuac-GGCGUC-GUGGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 19198 | 0.66 | 0.414769 |
Target: 5'- cCGCUCGGaucuccCAGC--GCUGC-GCugCCCg -3' miRNA: 3'- -GCGAGCC------GUUGuaCGGCGuCGugGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 32854 | 0.66 | 0.412885 |
Target: 5'- aCGCUUGcCAGCAgcuggauguccgGCCacaccgGCAGCGCCCa -3' miRNA: 3'- -GCGAGCcGUUGUa-----------CGG------CGUCGUGGGg -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 27286 | 0.67 | 0.387074 |
Target: 5'- cCGcCUCGGCAGCGcGuuGCucGGuCGCCgCCu -3' miRNA: 3'- -GC-GAGCCGUUGUaCggCG--UC-GUGG-GG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 14366 | 0.67 | 0.360625 |
Target: 5'- gGCUCGGgAACugcGCCuGCAGguCCgCg -3' miRNA: 3'- gCGAGCCgUUGua-CGG-CGUCguGGgG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 36407 | 0.67 | 0.3693 |
Target: 5'- cCGgaCGGCcGCAUGCC-CAGCGCa-- -3' miRNA: 3'- -GCgaGCCGuUGUACGGcGUCGUGggg -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 36697 | 0.67 | 0.378117 |
Target: 5'- gCGC-CGGCcuCAUGCgGCgcuGGCACCg- -3' miRNA: 3'- -GCGaGCCGuuGUACGgCG---UCGUGGgg -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 29738 | 0.67 | 0.387074 |
Target: 5'- cCGCcacuacCGGCAGCcgcaccUGCCGCaccGGCACCgCu -3' miRNA: 3'- -GCGa-----GCCGUUGu-----ACGGCG---UCGUGGgG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 27020 | 0.67 | 0.390696 |
Target: 5'- uGCUCGGCcu--UGUCGCcuacguccuggacagAGCcCCCCa -3' miRNA: 3'- gCGAGCCGuuguACGGCG---------------UCGuGGGG- -5' |
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30424 | 3' | -58.8 | NC_006548.1 | + | 26827 | 0.67 | 0.343705 |
Target: 5'- gCGCgaggauugCGGCAACcccauUGCCGUAGU-CCUCu -3' miRNA: 3'- -GCGa-------GCCGUUGu----ACGGCGUCGuGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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