Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30442 | 5' | -50.8 | NC_006548.1 | + | 13695 | 0.7 | 0.599371 |
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Target: 5'- uUCGccAGGCGuGCcuggucUUGGCUUGCGAAUCg -3' miRNA: 3'- -AGU--UCCGCuUGu-----AGCCGGACGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 18814 | 0.71 | 0.545847 |
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Target: 5'- aUCAGGGUguuggauuuggcgucGAGCAgcaCGGCCUGCGGc-- -3' miRNA: 3'- -AGUUCCG---------------CUUGUa--GCCGGACGUUuag -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 1668 | 0.71 | 0.529841 |
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Target: 5'- cCGGGGCGGAgagaguUAUCGGCUggGCAcAUCg -3' miRNA: 3'- aGUUCCGCUU------GUAGCCGGa-CGUuUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 28151 | 0.71 | 0.518514 |
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Target: 5'- --cAGGCGGuugAguUCGGCCUGCAGGc- -3' miRNA: 3'- aguUCCGCU---UguAGCCGGACGUUUag -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 15240 | 0.72 | 0.482957 |
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Target: 5'- gCAGGGCGAacucguccaggauGCGUccgccgcgcuugcCGGCCUGCGcGUCc -3' miRNA: 3'- aGUUCCGCU-------------UGUA-------------GCCGGACGUuUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 15047 | 0.73 | 0.452836 |
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Target: 5'- uUCAAGGa--ACAggCGcGCCUGCAGGUCa -3' miRNA: 3'- -AGUUCCgcuUGUa-GC-CGGACGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 3485 | 0.73 | 0.442329 |
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Target: 5'- aCAGGGUGAcGCgAUCGaCCUGCAGAUUg -3' miRNA: 3'- aGUUCCGCU-UG-UAGCcGGACGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 17189 | 1.12 | 0.001023 |
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Target: 5'- gUCAAGGCGAACAUCGGCCUGCAAAUCa -3' miRNA: 3'- -AGUUCCGCUUGUAGCCGGACGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 19283 | 0.66 | 0.843007 |
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Target: 5'- cCAAGGCGugcCGUCaggcgauaccugGGCCUG-GAAUCg -3' miRNA: 3'- aGUUCCGCuu-GUAG------------CCGGACgUUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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